ArticleBMC microbiology2009
Rapid identification of bacterial pathogens using a PCR- and microarray-based assay.
Article in BMC microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Modular RNA:DNA Nanostructures Enable Nanopore Profiling of rRNA Processing and rRNA Variants.ACS nano · 2026Article
- BioInnovate AI: A Machine Learning Platform for Rapid PCR Assay Design in Emerging Infectious Disease Diagnostics.Diagnostics (Basel, Switzerland) · 2025Article
- From Tradition to Innovation: Diverse Molecular Techniques in the Fight Against Infectious Diseases.Diagnostics (Basel, Switzerland) · 2024Review
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- Potential Application of the WST-8-mPMS Assay for Rapid Viable Microorganism Detection.Pathogens (Basel, Switzerland) · 2023Article
- Airborne transmission of biological agents within the indoor built environment: a multidisciplinary review.Air quality, atmosphere, & health · 2023Review
- Multiplex detection of five common respiratory pathogens from bronchoalveolar lavages using high resolution melting curve analysis.BMC microbiology · 2022Article
- Applications of Nanozymology in the Detection and Identification of Viral, Bacterial and Fungal Pathogens.International journal of molecular sciences · 2022Review
- Rapid Discrimination of Clinically Important Pathogens Through Machine Learning Analysis of Surface Enhanced Raman Spectra.Frontiers in microbiology · 2022Article
- Detection of Microorganisms in Body Fluids via MTT-PMS Assay.Diagnostics (Basel, Switzerland) · 2021Article
- MappingFoods (Basel, Switzerland) · 2021Article
- Study on bacterial pathogens through multiplex polymerase chain reaction system and their antimicrobial resistance pattern in goats presumed with fever and/or diarrhea.Veterinary world · 2021Article
- Imaging, Identification and Inhibition of Microorganisms Using AIEgens.Topics in current chemistry (Cham) · 2021Review
- Approaches for characterizing and tracking hospital-associated multidrug-resistant bacteria.Cellular and molecular life sciences : CMLS · 2021Review
- Microbial Electrochemical Systems: Principles, Construction and Biosensing Applications.Sensors (Basel, Switzerland) · 2021Review
- Suppurative necrotizing bronchopneumonia caused by Nocardia cyriacigeorgica infection in a stranded striped dolphin (Stenella coeruleoalba) in Japan.The Journal of veterinary medical science · 2021Article
- Visualization of endogenous gut bacteria in Drosophila melanogaster using fluorescence in situ hybridization.PloS one · 2021Article
- Recognition of (Sesc) for Easy Identification ofReports of biochemistry & molecular biology · 2020Article
- Direct Detection and Discrimination of Nucleotide Polymorphisms Using Anthraquinone Labeled DNA Probes.Frontiers in chemistry · 2020Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDuring the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. We have developed a method based on a modified broad-range PCR and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. The broad-range PCR primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrB and parE. The primer design allowed the use of a novel DNA amplification method, which produced labeled, single-stranded DNA suitable for microarray hybridization. The probes on the microarray were designed from the alignments of species- or genus-specific variable regions of the gyrB and parE genes flanked by the primers. We included mecA-specific primers and probes in the same assay to indicate the presence of methicillin resistance in the bacterial species. The feasibility of this assay in routine diagnostic testing was evaluated using 146 blood culture positive and 40 blood culture negative samples.
resultsComparison of our results with those of a conventional culture-based method revealed a sensitivity of 96% (initial sensitivity of 82%) and specificity of 98%. Furthermore, only one cross-reaction was observed upon investigating 102 culture isolates from 70 untargeted bacteria. The total assay time was only three hours, including the time required for the DNA extraction, PCR and microarray steps in sequence.
conclusionThe assay rapidly provides reliable data, which can guide optimal antimicrobial treatment decisions in a timely manner.
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