ArticleInvestigative ophthalmology & visual science2010
Perimysial fibroblasts of extraocular muscle, as unique as the muscle fibers.
Article in Investigative ophthalmology & visual science, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Extreme Tolerance of Extraocular Muscles to Diseases and Aging: Why and How?International journal of molecular sciences · 2024Review
- Mitochondrial bioenergetics in ocular fibroblasts of two myasthenia gravis cases.IBRO neuroscience reports · 2022Article
- Transcriptome and epigenome diversity and plasticity of muscle stem cells following transplantation.PLoS genetics · 2020Article
- Lipopolysaccharides promote pulmonary fibrosis in silicosis through the aggravation of apoptosis and inflammation in alveolar macrophages.Open life sciences · 2020Article
- Signals from the Niche: Insights into the Role of IGF-1 and IL-6 in Modulating Skeletal Muscle Fibrosis.Cells · 2019Review
- Potential Roles of CD34+ Fibrocytes Masquerading as Orbital Fibroblasts in Thyroid-Associated Ophthalmopathy.The Journal of clinical endocrinology and metabolism · 2019Review
- Using Whole Genome Sequencing in an African Subphenotype of Myasthenia Gravis to Generate a Pathogenetic Hypothesis.Frontiers in genetics · 2019Article
- Composition, Architecture, and Functional Implications of the Connective Tissue Network of the Extraocular Muscles.Investigative ophthalmology & visual science · 2018Article
- Altered Protein Composition and Gene Expression in Strabismic Human Extraocular Muscles and Tendons.Investigative ophthalmology & visual science · 2016Article
- RNA expression analysis of passive transfer myasthenia supports extraocular muscle as a unique immunological environment.Investigative ophthalmology & visual science · 2014Article
- Structure and function of the skeletal muscle extracellular matrix.Muscle & nerve · 2011Review
- Satellite cells, connective tissue fibroblasts and their interactions are crucial for muscle regeneration.Development (Cambridge, England) · 2011Article
- Development of extraocular muscles requires early signals from periocular neural crest and the developing eye.Archives of ophthalmology (Chicago, Ill. : 1960) · 2011Article
- Postnatal changes in the developing rat extraocular muscles.Investigative ophthalmology & visual science · 2011Article
- Connective tissue fibroblasts and Tcf4 regulate myogenesis.Development (Cambridge, England) · 2011Article
- Effects of recession versus tenotomy surgery without recession in adult rabbit extraocular muscle.Investigative ophthalmology & visual science · 2010Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
purposeExtraocular muscle (EOM) has a distinct skeletal muscle phenotype. The hypothesis for the study was that fibroblasts support the unique EOM phenotype and that perimysial fibroblasts derived from EOM have properties that distinguish them from fibroblasts derived from other skeletal muscle.
methodsPerimysial fibroblasts from leg muscle (LM-Fibro) and EOM (EOM-Fibro) of mice were derived and maintained in culture. EOM- and LM-Fibro were assessed morphologically and for vimentin, smooth muscle actin, and Thy-1 immunoreactivity. DNA microarray analysis was performed on LM- and EOM-Fibro grown in conditions that support myoblast differentiation. To assess trophic interactions, co-cultures of myoblasts from established cell lines, CL-EOM and CL-LM with, EOM- or LM-Fibro were performed in direct contact and in a permeable filter support culture. The degree of myotube maturation was assessed by the percentage of myotubes with more than three myonuclei per myotube.
resultsEOM- and LM-Fibro cells exhibited distinct morphologies. Both cell types proliferated as a monolayer and expressed vimentin. Fifty-five percent (SD 4.4%) of EOM-Fibro were Thy-1 positive compared with only 24% (SD 4.4%) of LM-Fibro. DNA microarray analysis demonstrated differential expression of structural, immune response, and metabolism-related genes between EOM- and LM-Fibro. Co-cultures demonstrated that mature myotube formation in EOM-derived cell lines was supported to a greater extent by EOM-Fibro than by LM-Fibro, compared with CL-EOM grown with LM-Fibro.
conclusionsFibroblasts from EOM demonstrate distinct properties that distinguish them from leg muscle-derived fibroblasts. The distinct properties of EOM-Fibro may support the unique EOM phenotype and contribute to their differential involvement in disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.