ArticleJournal of immunological methods2009
A method for isolating and analyzing human mRNA from newborn stool.
Article in Journal of immunological methods, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed.
- Optimizing protocols for microRNA profiling of infant and toddler stool.RNA biology · 2026Article
- Intestinal Inflammation in Chilean Infants Fed With Bovine Formula vs. Breast Milk and Its Association With Their Gut Microbiota.Frontiers in cellular and infection microbiology · 2018Article
- Fecal Host Transcriptomics for Non-Invasive Human Mucosal Immune Profiling: Proof of Concept inPathogens & immunity · 2018Article
- Droplet digital PCR quantifies host inflammatory transcripts in feces reliably and reproducibly.Cellular immunology · 2016Article
- Detection of spliced mRNA from human bocavirus 1 in clinical samples from children with respiratory tract infections.Emerging infectious diseases · 2013Article
- MAPK-activated protein kinase 2 contributes to Clostridium difficile-associated inflammation.Infection and immunity · 2013Article
- Generation and analysis of a mouse intestinal metatranscriptome through Illumina based RNA-sequencing.PloS one · 2012Article
- Proinflammatory fecal mRNA and childhood bacterial enteric infections.Gut microbes · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Efforts to characterize the human transcriptome have largely been limited to blood, urine, and tissue analyses (i.e., normally sterile materials). We report here an extraction protocol using commercially available reagents to obtain high-yield, reverse-transcribable RNA from human stool. Quantitative reverse transcriptase polymerase chain reactions demonstrated minimal intra-specimen but considerable intra-subject variability over time of transcripts for interleukin-6 (IL-6), IL-8, epidermal growth factor (EGF), calprotectin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). This technique now expands opportunities to use the human fecal transcriptome to characterize gastrointestinal pathophysiology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.