ArticleFEMS microbiology letters2009
Mini-Tn7 vectors as genetic tools for gene cloning at a single copy number in an industrially important and phytopathogenic bacteria, Xanthomonas spp.
Article in FEMS microbiology letters, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 36 citations in OpenAlex.
- The RpfF Acyl-ACP thioesterase of major rice pathogen Xanthomonas oryzae pv. oryzae is essential for maintenance of membrane function and virulence.PLoS pathogens · 2026Article
- The phytohormone indole-3-acetic acid induces quorum sensing signal DSF turnover via a positive feedback biosynthetic loop in the phytopathogen Xanthomonas campestris.Stress biology · 2026Article
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- Improved mini-TnApplied and environmental microbiology · 2023Article
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- A versatile TnPlant communications · 2022Article
- An improved, versatile and efficient modular plasmid assembly system for expression analyses of genes in Xanthomonas oryzae.Molecular plant pathology · 2021Article
- Biological Functions ofFrontiers in microbiology · 2017Article
- Mini-Tn7 Insertion in an Artificial attTn7 Site Enables Depletion of the Essential Master Regulator CtrA in the Phytopathogen Agrobacterium tumefaciens.Applied and environmental microbiology · 2016Article
- Mini-Tn7 vectors for stable expression of diguanylate cyclase PleD* in Gram-negative bacteria.BMC microbiology · 2015Article
- Regulation by SoxR of mfsA, Which Encodes a Major Facilitator Protein Involved in Paraquat Resistance in Stenotrophomonas maltophilia.PloS one · 2015Article
- Construction of mobilizable mini-Tn7 vectors for bioluminescent detection of gram-negative bacteria and single-copy promoter lux reporter analysis.Applied and environmental microbiology · 2013Article
- Construction of a broad-host-range Tn7-based vector for single-copy P(BAD)-controlled gene expression in gram-negative bacteria.Applied and environmental microbiology · 2013Article
- Evaluation of the virulence of Xanthomonas campestris pv. campestris mutant strains lacking functional genes in the OxyR regulon.Current microbiology · 2011Article
- The catalase-peroxidase KatG is required for virulence of Xanthomonas campestris pv. campestris in a host plant by providing protection against low levels of H2O2.Journal of bacteriology · 2009Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
Transposon mini-Tn7 vectors insert into the chromosome of several Gram-negative bacteria in a site-specific manner. Here, we showed the application of mini-Tn7 as single copy site-specific integration vector system for Xanthomonas campestris pv. campestris. The transposition of the mini-Tn7 into the bacterial genome was detected at a Tn7 attachment (attTn7) site located downstream of glmS1. Furthermore, using a newly constructed vector pBBR1FLP2 containing the flipase (FLP) recombinase for site-specific excision of the sequence between the FLP recombinase target (FRT) sites, and a sacB counter selection marker, an unmarked mini-Tn7 insertion mutant was created. Mini-Tn7 insertion did not affect bacterial virulence on the tested plant. The mini-Tn7 and FLP-FRT systems also work well in Xanthomonas oryzae pv. oryzae.
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