ArticleAmerican journal of physiology. Cell physiology2009
The mechanism of opiorphin-induced experimental priapism in rats involves activation of the polyamine synthetic pathway.
Article in American journal of physiology. Cell physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 52 citations in OpenAlex.
- An update on mechanisms and treatment options for priapism.Nature reviews. Urology · 2025Review
- Idiopathic recurrent ischemic priapism: a review of current literature and an algorithmic approach to evaluation and management.Basic and clinical andrology · 2024Article
- Opiorphin: an endogenous human peptide with intriguing application in diverse range of pathologies.Inflammopharmacology · 2024Review
- Article
- Using cyproterone acetate to treat recurrent ischemic priapism in a patient with sickle cell anemia as a comorbidity: a case report.Journal of medical case reports · 2020Article
- Clinical and Genetic Predictors of Priapism in Sickle Cell Disease: Results from the Recipient Epidemiology and Donor Evaluation Study III Brazil Cohort Study.The journal of sexual medicine · 2019Article
- Beneficial Effect of the Nitric Oxide Donor Compound 3-(1,3-Dioxoisoindolin-2-yl)Benzyl Nitrate on Dysregulated Phosphodiesterase 5, NADPH Oxidase, and Nitrosative Stress in the Sickle Cell Mouse Penis: Implication for Priapism Treatment.The Journal of pharmacology and experimental therapeutics · 2016Article
- Effect of ischaemic brain injury on sexual function in adult mice.Stroke and vascular neurology · 2016Article
- Subacute Hemolysis in Sickle Cell Mice Causes Priapism Secondary to NO Imbalance and PDE5 Dysregulation.The journal of sexual medicine · 2015Article
- Opiorphin-dependent upregulation of CD73 (a key enzyme in the adenosine signaling pathway) in corporal smooth muscle cells exposed to hypoxic conditions and in corporal tissue in pre-priapic sickle cell mice.International journal of impotence research · 2015Article
- Nitrergic Mechanisms for Management of Recurrent Priapism.Sexual medicine reviews · 2015Article
- How I treat priapism.Blood · 2015Article
- Is testosterone deficiency a possible risk factor for priapism associated with sickle-cell disease?International urology and nephrology · 2015Article
- Molecular pathophysiology of priapism: emerging targets.Current drug targets · 2015Review
- Management of priapism: an update for clinicians.Therapeutic advances in urology · 2014Review
- Opiorphin is a master regulator of the hypoxic response in corporal smooth muscle cells.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
- Sildenafil promotes eNOS activation and inhibits NADPH oxidase in the transgenic sickle cell mouse penis.The journal of sexual medicine · 2014Article
- Molecular analysis of erection regulatory factors in sickle cell disease associated priapism in the human penis.The Journal of urology · 2013Article
- Article
- A pathophysiology-based approach to the management of early priapism.Asian journal of andrology · 2013Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Intracorporal injection of plasmids encoding opiorphins into retired breeder rats can result in animals developing a priapic-like condition. Microarray analysis demonstrated that following intracorporal gene transfer of plasmids expressing opiorphins the most significantly upregulated gene in corporal tissue was the ornithine decarboxylase gene (ODC). Quantitative RT-PCR confirmed the upregulation of ODC, as well as other genes involved in polyamine synthesis, such as arginase-I and -II, polyamine oxidase, spermidine synthase, spermidine acetyltransferase (SAT), and S-adenosylmethionine decarboxylase. Western blot analysis demonstrated upregulation of arginase-I and -II, ODC, and SAT at the protein level. Levels of the polyamine putrescine were upregulated in animals treated with opiorphin-expressing plasmids compared with controls. A direct role for the upregulation of polyamine synthesis in the development of the priapic-like condition was supported by the observation that the ODC inhibitor 1,3-diaminopropane, when added to the drinking water of animals treated with plasmids expressing opiorphins, prevented experimental priapism. We also demonstrate that in sickle cell mice, another model of priapism, there is increased expression of the mouse opiorphin homologue in corporal tissue compared with the background strain at a life stage prior to evidence of priapism. At a life stage when there is onset of priapism, there is increased expression of the enzymes involved in polyamine synthesis (ODC and arginase-I and -II). Our results suggest that the upregulation of enzymes involved in the polyamine synthetic pathway may play a role in the development of experimental priapism and represent a target for the prevention of priapism.
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