ArticleAmerican journal of physiology. Renal physiology2009
Mechanoregulation of BK channel activity in the mammalian cortical collecting duct: role of protein kinases A and C.
Article in American journal of physiology. Renal physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 46 citations in OpenAlex.
- Going with the flow: New insights regarding flow induced KPhysiological reports · 2024Review
- TRPV4 expression in the renal tubule is necessary for maintaining whole body KAmerican journal of physiology. Renal physiology · 2023Article
- L-WNK1 is required for BK channel activation in intercalated cells.American journal of physiology. Renal physiology · 2021Article
- Antiproteinuric and Hyperkalemic Mechanisms Activated by Dual Versus Single Blockade of the RAS in Renovascular Hypertensive Rats.Frontiers in physiology · 2021Article
- Intercalated cell BKα subunit is required for flow-induced K+ secretion.JCI insight · 2020Article
- Discovery, Characterization, and Effects on Renal Fluid and Electrolyte Excretion of the Kir4.1 Potassium Channel Pore Blocker, VU0134992.Molecular pharmacology · 2018Article
- NADPH oxidase 4-derived superoxide mediates flow-stimulated NKCC2 activity in thick ascending limbs.American journal of physiology. Renal physiology · 2018Article
- Molecular Mechanisms Underlying Renin-Angiotensin-Aldosterone System Mediated Regulation of BK Channels.Frontiers in physiology · 2017Review
- The mechanosensitive BKα/β1 channel localizes to cilia of principal cells in rabbit cortical collecting duct (CCD).American journal of physiology. Renal physiology · 2017Article
- Maintaining KAmerican journal of physiology. Renal physiology · 2016Review
- An unexpected journey: conceptual evolution of mechanoregulated potassium transport in the distal nephron.American journal of physiology. Cell physiology · 2016Review
- Article
- Cell-specific regulation of L-WNK1 by dietary K.American journal of physiology. Renal physiology · 2016Article
- Chemical and Physical Sensors in the Regulation of Renal Function.Clinical journal of the American Society of Nephrology : CJASN · 2015Review
- Cholesterol affects flow-stimulated cyclooxygenase-2 expression and prostanoid secretion in the cortical collecting duct.American journal of physiology. Renal physiology · 2015Article
- WNK1 activates large-conductance Ca2+-activated K+ channels through modulation of ERK1/2 signaling.Journal of the American Society of Nephrology : JASN · 2015Article
- Distal convoluted tubule.Comprehensive Physiology · 2015Review
- Effects of biomechanical forces on signaling in the cortical collecting duct (CCD).American journal of physiology. Renal physiology · 2014Article
- Interacting influence of diuretics and diet on BK channel-regulated K homeostasis.Current opinion in pharmacology · 2014Review
- WNK4 inhibits Ca(2+)-activated big-conductance potassium channels (BK) via mitogen-activated protein kinase-dependent pathway.Biochimica et biophysica acta · 2013Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Flow-stimulated net K secretion (J(K)) in the cortical collecting duct (CCD) is mediated by an iberiotoxin (IBX)-sensitive BK channel, and requires an increase in intracellular Ca2+ concentration ([Ca2+](i)). The alpha-subunit of the reconstituted BK channel is phosphorylated by PKA and PKC. To test whether the BK channel in the native CCD is regulated by these kinases, J(K) and net Na absorption (J(Na)) were measured at slow (approximately 1) and fast (approximately 5 nl x min(-1) x mm(-1)) flow rates in rabbit CCDs microperfused in the presence of mPKI, an inhibitor of PKA; calphostin C, which inhibits diacylglycerol binding proteins, including PKC; or bisindolylmaleimide (BIM) and Gö6976, inhibitors of classic and novel PKC isoforms, added to luminal (L) and/or basolateral (B) solutions. L but not B mPKI increased J(K) in CCDs perfused at a slow flow rate; a subsequent increase in flow rate augmented J(K) modestly. B mPKI alone or with L inhibitor abolished flow stimulation of J(K). Similarly, L calphostin C increased J(K) in CCDs perfused at slow flow rates, as did calphostin C in both L and B solutions. The observation that IBX inhibited the L mPKI- and calphostin C-mediated increases in J(K) at slow flow rates implicated the BK channel in this K flux, a notion suggested by patch-clamp analysis of principal cells. The kinase inhibited by calphostin C was not PKC as L and/or B BIM and Gö6976 failed to enhance J(K) at the slow flow rate. However, addition of these PKC inhibitors to the B solution alone or with L inhibitor blocked flow stimulation of J(K). Interpretation of these results in light of the effects of these inhibitors on the flow-induced elevation of [Ca2+](i) suggests that the principal cell apical BK channel is tonically inhibited by PKA and that flow stimulation of J(K) in the CCD is PKA and PKC dependent. The specific targets of the kinases remain to be identified.
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