ArticleJournal of virology2009
Reconstitution of authentic nanovirus from multiple cloned DNAs.
Article in Journal of virology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed, 72 citations in OpenAlex.
- Small impact of the host plant physiological state on the genome formula of a nanovirus.PLoS pathogens · 2026Article
- Review
- Construction and Segmental Reconstitution of Full-Length Infectious Clones of Milk Vetch Dwarf Virus.Viruses · 2025Article
- Replication-independent change in the frequencies of distinct genome segments of a multipartite virus during its transit within aphid vectors.Microbiology spectrum · 2024Article
- The genome formula of a multipartite virus is regulated both at the individual segment and the segment group levels.PLoS pathogens · 2024Article
- Reassortments in single-stranded DNA multipartite viruses: Confronting expectations based on molecular constraints with field observations.Virus evolution · 2024Review
- Structure-guided mutagenesis of the capsid protein indicates that a nanovirus requires assembled viral particles for systemic infection.PLoS pathogens · 2023Article
- Assembly of plant virus agroinfectious clones using biological material or DNA synthesis.STAR protocols · 2022Article
- Nonconcomitant host-to-host transmission of multipartite virus genome segments may lead to complete genome reconstitution.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Effects of an Alphasatellite on the Life Cycle of the NanovirusJournal of virology · 2022Article
- Viruses Infecting Trees and Herbs That Produce Edible Fleshy Fruits with a Prominent Value in the Global Market: An Evolutionary Perspective.Plants (Basel, Switzerland) · 2022Review
- Gene copy number variations at the within-host population level modulate gene expression in a multipartite virus.Virus evolution · 2022Article
- Banana bunchy top virus genetic diversity in Pakistan and association of diversity with recombination in its genomes.PloS one · 2022Article
- ICTV Virus Taxonomy Profile:The Journal of general virology · 2021Article
- Route of a Multipartite Nanovirus across the Body of Its Aphid Vector.Journal of virology · 2020Article
- Co-Acquired Nanovirus and Geminivirus Exhibit a Contrasted Localization within Their Common Aphid Vector.Viruses · 2020Article
- Nanovirus Disease Complexes: An Emerging Threat in the Modern Era.Frontiers in plant science · 2020Review
- Phosphorylations of the Abutilon Mosaic Virus Movement Protein Affect Its Self-Interaction, Symptom Development, Viral DNA Accumulation, and Host Range.Frontiers in plant science · 2020Article
- Home-made enzymatic premix and Illumina sequencing allow for one-step Gibson assembly and verification of virus infectious clones.Phytopathology research · 2020Article
- Localizing Genome Segments and Protein Products of a Multipartite Virus in Host Plant Cells.Bio-protocol · 2019Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe a new plant single-stranded DNA (ssDNA) virus, a nanovirus isolate originating from the faba bean in Ethiopia. We applied rolling circle amplification (RCA) to extensively copy the individual circular DNAs of the nanovirus genome. By sequence analyses of more than 208 individually cloned genome components, we obtained a representative sample of eight polymorphic swarms of circular DNAs, each about 1 kb in size. From these heterogeneous DNA populations after RCA, we inferred consensus sequences of the eight DNA components of the virus genome. Based on the distinctive molecular and biological properties of the virus, we propose to consider it a new species of the genus Nanovirus and to name it faba bean necrotic stunt virus (FBNSV). Selecting a representative clone of each of the eight DNAs for transfer by T-DNA plasmids of Agrobacterium tumefaciens into Vicia faba plants, we elicited the development of the typical FBNSV disease symptoms. Moreover, we showed that the virus thus produced was readily transmitted by two different aphid vector species, Aphis craccivora and Acyrthosiphon pisum. This represents the first reconstitution of a fully infectious and sustainably insect-transmissible nanovirus from its cloned DNAs and provides compelling evidence that the genome of a legume-infecting nanovirus is typically comprised of eight distinct DNA components.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.