ArticleGenes & development2009
Sequence, biogenesis, and function of diverse small RNA classes bound to the Piwi family proteins of Tetrahymena thermophila.
Article in Genes & development, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
60 citing papers in PubMed, 83 citations in OpenAlex.
- Conserved but mechanistically diverse piRNA defence against endogenous retroviruses in insects.EMBO reports · 2026Article
- The Role of Membrane-Bound Extracellular Vesicles During Co-Stimulation and Conjugation in the CiliateMicroorganisms · 2025Article
- tRNA-derived small RNAs (tsRNAs) in cardiovascular diseases: biogenesis, functions, and therapeutic targets.Frontiers in cardiovascular medicine · 2025Review
- Regulation of insect behavior by non-coding RNAs.Science China. Life sciences · 2024Review
- Multiple regulatory roles of the transfer RNA-derived small RNAs in cancers.Genes & diseases · 2024Review
- tRNA renovatio: Rebirth through fragmentation.Molecular cell · 2023Review
- An old friend with a new face: tRNA-derived small RNAs with big regulatory potential in cancer biology.British journal of cancer · 2023Review
- Developmental mRNA clearance by PIWI-bound endo-siRNAs in Paramecium.Cell reports · 2023Article
- The epigenetic regulatory mechanism of PIWI/piRNAs in human cancers.Molecular cancer · 2023Review
- Review
- PIWI-Directed DNA Elimination for Tetrahymena Genetics.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Distinct Cell-specific Roles of NOX2 and MyD88 in Epileptogenesis.Frontiers in cell and developmental biology · 2022Review
- Small RNA-directed DNA elimination: the molecular mechanism and its potential for genome editing.RNA biology · 2021Review
- Disruption of a ∼23-24 nucleotide small RNA pathway elevates DNA damage responses inMolecular biology of the cell · 2021Article
- Roles of piRNAs in transposon and pseudogene regulation of germline mRNAs and lncRNAs.Genome biology · 2021Review
- Transfer RNA-Derived Small RNAs: Another Layer of Gene Regulation and Novel Targets for Disease Therapeutics.Molecular therapy : the journal of the American Society of Gene Therapy · 2020Review
- tRNA-Derived Small RNAs: Novel Epigenetic Regulators.Cancers · 2020Review
- On the expanding roles of tRNA fragments in modulating cell behavior.Nucleic acids research · 2020Review
- Action mechanisms and research methods of tRNA-derived small RNAs.Signal transduction and targeted therapy · 2020Review
- Characterization of Simple Sequence Repeats (SSRs) in Ciliated Protists Inferred by Comparative Genomics.Microorganisms · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PAZ/PIWI domain (PPD) proteins carrying small RNAs (sRNAs) function in gene and genome regulation. The ciliate Tetrahymena thermophila encodes numerous PPD proteins exclusively of the Piwi clade. We show that the three Tetrahymena Piwi family proteins (Twis) preferentially expressed in growing cells differ in their genetic essentiality and subcellular localization. Affinity purification of all eight distinct Twi proteins revealed unique properties of their bound sRNAs. Deep sequencing of Twi-bound and total sRNAs in strains disrupted for various silencing machinery uncovered an unanticipated diversity of 23- to 24-nt sRNA classes in growing cells, each with distinct genetic requirements for accumulation. Altogether, Twis distinguish sRNAs derived from loci of pseudogene families, three types of DNA repeats, structured RNAs, and EST-supported loci with convergent or paralogous transcripts. Most surprisingly, Twi7 binds complementary strands of unequal length, while Twi10 binds a specific permutation of the guanosine-rich telomeric repeat. These studies greatly expand the structural and functional repertoire of endogenous sRNAs and RNPs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.