ArticleThe Journal of endocrinology2009
The bovine mammary gland expresses multiple functional isoforms of serotonin receptors.
Article in The Journal of endocrinology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 64 citations in OpenAlex.
- Article
- Zeolite for preventing periparturient hypocalcemia in dairy cows: mechanisms and application strategies.Frontiers in veterinary science · 2025Review
- Exploring the contribution of mammary-derived serotonin on liver and pancreas metabolism during lactation.PloS one · 2024Article
- 5-Hydroxytryptophan inhibits β-casein biosynthesis and promotes goat mammary epithelial cell apoptosis through the JAK2/STAT5a axis and the HTR7.Journal of animal science · 2023Article
- Effect of serotonin on the cell viability of the bovine mammary alveolar cell-T (MAC-T) cell line.Journal of animal science and technology · 2022Article
- In vitro effects of 5-Hydroxy-L-tryptophan supplementation on primary bovine mammary epithelial cell gene expression under thermoneutral or heat shock conditions.Scientific reports · 2022Article
- Impact of Fluoxetine Treatment and Folic Acid Supplementation on the Mammary Gland Transcriptome During Peak Lactation.Frontiers in pharmacology · 2022Article
- Elevated serotonin coordinates mammary metabolism in dairy cows.Physiological reports · 2021Article
- Serotonin regulates maternal calcium homeostasis during the perinatal period of sheep.Journal of animal science · 2019Article
- Could use of Selective Serotonin Reuptake Inhibitors During Lactation Cause Persistent Effects on Maternal Bone?Journal of mammary gland biology and neoplasia · 2018Review
- Serotonin induces parathyroid hormone-related protein in goat mammary gland.Journal of animal science · 2018Article
- Serum metabolomic profile in genetically modified cows carrying human α‑lactalbumin gene.Molecular medicine reports · 2017Article
- TRIENNIAL LACTATION SYMPOSIUM/BOLFA: Serotonin and the regulation of calcium transport in dairy cows.Journal of animal science · 2017Review
- Characterization of mammary-specific disruptions for Tph1 and Lrp5 during murine lactation.Scientific reports · 2017Article
- Serotoninergic and Circadian Systems: Driving Mammary Gland Development and Function.Frontiers in physiology · 2016Article
- Transcriptomic Analysis of the Mouse Mammary Gland Reveals New Insights for the Role of Serotonin in Lactation.PloS one · 2015Article
- The type 7 serotonin receptor, 5-HT 7 , is essential in the mammary gland for regulation of mammary epithelial structure and function.BioMed research international · 2015Article
- Peripheral serotonin regulates maternal calcium trafficking in mammary epithelial cells during lactation in mice.PloS one · 2014Article
- Bovine mammary epithelial cell cultures for the study of mammary gland functions.In vitro cellular & developmental biology. Animal · 2014Article
- Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Recent studies in dairy cows have demonstrated that serotonergic ligands affect milk yield and composition. Correspondingly, serotonin (5-HT) has been demonstrated to be an important local regulator of lactational homeostasis and involution in mouse and human mammary cells. We determined the mRNA expression of bovine 5-HT receptor (HTR) subtypes in bovine mammary tissue (BMT) and used pharmacological agents to evaluate functional activities of 5-HT receptors. The mRNAs for five receptor isoforms (HTR1B, 2A, 2B, 4, and 7) were identified by conventional real-time (RT)-PCR, RT quantitative PCR, and in situ hybridization in BMT. In addition to luminal mammary epithelial cell expression, HTR4 was expressed in myoepithelium, and HTR1B, 2A, and 2B were expressed in small mammary blood vessels. Serotonin suppressed milk protein mRNA expression (alpha-lactalbumin and beta-casein mRNA) in lactogen-treated primary bovine mammary epithelial cell (BMEC) cultures. To probe the functional activities of individual receptors, caspase-3 activity and expression of alpha-lactalbumin and beta-casein were measured. Both SB22489 (1B antagonist) and ritanserin (2A antagonist) increased caspase-3 activity. Expression of alpha-lactalbumin and beta-casein mRNA levels in BMEC were stimulated by low concentrations of SB224289, ritanserin, or pimozide. These results demonstrate that there are multiple 5-HT receptor isoforms in the bovine mammary gland, and point to profound differences between serotonergic systems of the bovine mammary gland and the human and mouse mammary glands. Whereas human and mouse mammary epithelial cells express predominately the protein for the 5-HT(7) receptor, cow mammary epithelium expresses multiple receptors that have overlapping, but not identical, functional activities.
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