ArticleProceedings of the National Academy of Sciences of the United States of America2009
The actin-like MreB cytoskeleton organizes viral DNA replication in bacteria.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 53 citations in OpenAlex.
- MreB: unraveling the molecular mechanisms of bacterial shape, division, and environmental adaptation.Cell communication and signaling : CCS · 2025Review
- Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.Cell host & microbe · 2025Article
- A transcriptionally active lipid vesicle encloses the injectedbioRxiv : the preprint server for biology · 2024Article
- The intricate organizational strategy of nucleus-forming phages.Current opinion in microbiology · 2024Review
- Replication Compartments of Eukaryotic and Bacterial DNA Viruses: Common Themes Between Different Domains of Host Cells.Annual review of virology · 2022Review
- Subcellular organization of viral particles during maturation of nucleus-forming jumbo phage.Science advances · 2022Article
- Structural and Functional Insight into the Mechanism ofNon-coding RNA · 2022Article
- Temporal compartmentalization of viral infection in bacterial cells.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Review
- The Phage Nucleus and PhuZ Spindle: Defining Features of the Subcellular Organization and Speciation of Nucleus-Forming Jumbo Phages.Frontiers in microbiology · 2021Review
- Emerging Aspects of Jumbo Bacteriophages.Infection and drug resistance · 2021Review
- Genomic Analysis of the Recent Viral Isolate vB_BthP-Goe4 Reveals Increased Diversity of φ29-Like Phages.Viruses · 2018Article
- Infection by bacteriophage lambda: an evolving paradigm for cellular individuality.Current opinion in microbiology · 2018Review
- Polar localization of MreB actin is inhibited by anionic phospholipids in the rod-shaped bacterium Escherichia coli.Current genetics · 2017Review
- Formation of a Viral Replication Focus in Sulfolobus Cells Infected by the Rudivirus Sulfolobus islandicus Rod-Shaped Virus 2.Journal of virology · 2017Article
- Jumbo Bacteriophages: An Overview.Frontiers in microbiology · 2017Article
- Natural transformation occurs independently of the essential actin-like MreB cytoskeleton in Legionella pneumophila.Scientific reports · 2015Article
- A prophage-encoded actin-like protein required for efficient viral DNA replication in bacteria.Nucleic acids research · 2015Article
- Revealing bacterial targets of growth inhibitors encoded by bacteriophage T7.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- The effect of inhibition of host MreB on the infection of thermophilic phage GVE2 in high temperature environment.Scientific reports · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Little is known about the organization or proteins involved in membrane-associated replication of prokaryotic genomes. Here we show that the actin-like MreB cytoskeleton of the distantly related bacteria Escherichia coli and Bacillus subtilis is required for efficient viral DNA replication. Detailed analyses of B. subtilis phage ϕ29 showed that the MreB cytoskeleton plays a crucial role in organizing phage DNA replication at the membrane. Thus, phage double-stranded DNA and components of the ϕ29 replication machinery localize in peripheral helix-like structures in a cytoskeleton-dependent way. Importantly, we show that MreB interacts directly with the ϕ29 membrane-protein p16.7, responsible for attaching viral DNA at the cell membrane. Altogether, the results reveal another function for the MreB cytoskeleton and describe a mechanism by which viral DNA replication is organized at the bacterial membrane.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.