ArticleBMC genomics2009
Brucella microti: the genome sequence of an emerging pathogen.
Article in BMC genomics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Two novel real-time PCR assays forMicrobiology spectrum · 2026Article
- Genomic and Immunoinformatics Insights Into a Bovine-Derived Brucella abortus S19 Field Strain: Adaptations Impacting Vaccine Efficacy.Veterinary medicine and science · 2025Article
- Brucella microti and Rodent-Borne Brucellosis: A Neglected Public Health Threat.Zoonoses and public health · 2025Review
- First isolation and identification ofFrontiers in microbiology · 2025Article
- Closely Related Brucella Species Widely Differ in their Vegetative and Intracellular Growth.Current microbiology · 2024Article
- Evidence of a Viable but Nonculturable (VBNC) Phase inMicroorganisms · 2024Article
- Mining the Flavoproteome of Brucella ovis, the Brucellosis Causing Agent in Ovis aries.Microbiology spectrum · 2022Article
- The Retrospective on AtypicalMicroorganisms · 2022Review
- High-Resolution Melting PCR as Rapid Genotyping Tool forMicroorganisms · 2022Article
- Comparative Genome-Wide Transcriptome Analysis ofFrontiers in microbiology · 2021Article
- Survival of Brucella abortus S19 and other Brucella spp. in the presence of oxidative stress and within macrophages.Folia microbiologica · 2020Article
- Laboratory Diagnosis of Human Brucellosis.Clinical microbiology reviews · 2019Review
- Brucellosis: Evolution and expected comeback.International journal of veterinary science and medicine · 2018Review
- Phenotypic and Molecular Characterization ofFrontiers in veterinary science · 2018Article
- Variability in the response of canine and human dendritic cells stimulated with Brucella canis.Veterinary research · 2017Article
- Chronic Brucella Infection Induces Selective and Persistent Interferon Gamma-Dependent Alterations of Marginal Zone Macrophages in the Spleen.Infection and immunity · 2017Article
- Article
- Brucella spp. of amphibians comprise genomically diverse motile strains competent for replication in macrophages and survival in mammalian hosts.Scientific reports · 2017Article
- Identification ofFrontiers in microbiology · 2017Article
- Transcriptome-Wide Identification of Hfq-Associated RNAs in Brucella suis by Deep Sequencing.Journal of bacteriology · 2016Article
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4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundUsing a combination of pyrosequencing and conventional Sanger sequencing, the complete genome sequence of the recently described novel Brucella species, Brucella microti, was determined. B. microti is a member of the genus Brucella within the Alphaproteobacteria, which consists of medically important highly pathogenic facultative intracellular bacteria. In contrast to all other Brucella species, B. microti is a fast growing and biochemically very active microorganism with a phenotype more similar to that of Ochrobactrum, a facultative human pathogen. The atypical phenotype of B. microti prompted us to look for genomic differences compared to other Brucella species and to look for similarities with Ochrobactrum.
resultsThe genome is composed of two circular chromosomes of 2,117,050 and 1,220,319 base pairs. Unexpectedly, we found that the genome sequence of B. microti is almost identical to that of Brucella suis 1330 with an overall sequence identity of 99.84% in aligned regions. The most significant structural difference between the two genomes is a bacteriophage-related 11,742 base pairs insert only present in B. microti. However, this insert is unlikely to have any phenotypical consequence. Only four protein coding genes are shared between B. microti and Ochrobactrum anthropi but impaired in other sequenced Brucella. The most noticeable difference between B. microti and other Brucella species was found in the sequence of the 23S ribosomal RNA gene. This unusual variation could have pleiotropic effects and explain the fast growth of B. microti.
conclusionContrary to expectations from the phenotypic analysis, the genome sequence of B. microti is highly similar to that of known Brucella species, and is remotely related to the one of O. anthropi. How the few differences in gene content between B. microti and B. suis 1330 could result in vastly different phenotypes remains to be elucidated. This unexpected finding will complicate the task of identifying virulence determinants in the Brucella genus. The genome sequence of B. microti will serve as a model for differential expression analysis and complementation studies. Our results also raise some concerns about the importance given to phenotypical traits in the definition of bacterial species.
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