Evidence map›Paper›PMID 19653309›Full record

ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2009

Improved cre reporter transgenic Xenopus.

Scott A Rankin, Takashi Hasebe, Aaron M Zorn, Daniel R Buchholz

Open access · bronzeAbstract read
In one paragraph

Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
1.6field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 26 citations in OpenAlex.

  1. Article
  2. Article
  3. Tbx5 driveseLife · 2021
    Article
  4. Frontiers in physiology · 2019
    Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Site-specific transgenesis in Xenopus.Genesis (New York, N.Y. : 2000) · 2012
    Article
  12. Review
  13. Article
  14. Review
  15. Article
  16. New doxycycline-inducible transgenic lines in Xenopus.Developmental dynamics : an official publication of the American Association of Anatomists · 2011
    Article
  17. Apoptosis in amphibian organs during metamorphosis.Apoptosis : an international journal on programmed cell death · 2010
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 2 countries.

Scott A RankinDivision of Developmental Biology, Cincinnati Children's Research Foundation and Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, Ohio, USA.
Takashi Hasebe
Aaron M Zorn
Daniel R Buchholz
Cincinnati Children's Hospital Medical Center · USNippon Medical School · JPUniversity of Cincinnati · US

Funding

Molecular Basis of Liver Development.R01DK070858 · NIDDK · CINCINNATI CHILDRENS HOSP MED CTR · PI ZORN, AARON M · 2007 to 2017
$2.9M
NIDDK NIH HHS DK070858NIDDK NIH HHS R01 DK070858
6 · The paper itself

Abstract

We have produced and characterized improved transgenic reporter lines for detection of Cre recombinase activity during Xenopus development. Improvements include choice of fluorophores, which make these Cre reporter lines generally suitable for lineage tracing studies. We also include data for several new parameters affecting survival and transgenesis efficiency using the recently developed meganuclease method of frog transgenesis. These transgenic frogs express cyan fluorescent protein (CFP) under control of the ubiquitous promoter CMV, where CFP is replaced by DsRed2 (a red fluorescent protein) in the presence of Cre. Three independent, high expression, Cre-sensitive lines have been identified that maintain robust fluorophore expression across generations and lack DsRed2 expression in the absence of Cre. A novel use of these lines is to indelibly mark embryonic blastomeres by Cre mRNA injection for permanent fate mapping. Similarly, transgenically expressed Cre under control of tissue-specific promoters will allow detailed analysis of cell lineage relationships throughout embryogenesis, metamorphosis, and adulthood.

Indexed as

AnimalsAnimals, Genetically ModifiedIntegrasesTransgenesXenopusCre recombinaseIntegrases

Identifiers

PMID19653309
PMCPMC2779334
OpenAlexW2079650929

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.