ArticlePLoS neglected tropical diseases2009
Burkholderia Pseudomallei is genetically diverse in agricultural land in Northeast Thailand.
Article in PLoS neglected tropical diseases, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it, 42 citations in OpenAlex.
- Systematic review and consensus guidelines for environmental sampling of Burkholderia pseudomallei.PLoS neglected tropical diseases · 2013Pooled it
- Trimethoprim-sulfamethoxazole versus trimethoprim-sulfamethoxazole plus doxycycline as oral eradicative treatment for melioidosis (MERTH): a multicentre, double-blind, non-inferiority, randomised controlled trial.Lancet (London, England) · 2014Trial
- Seasonal change of Burkholderia pseudomallei in paddy field water strongly correlates with ambient temperature: A study in north-central Vietnam.PLoS neglected tropical diseases · 2025Article
- Inhibitory effects ofPeerJ · 2025Article
- A systematic review and meta-analysis of the global prevalence and relationships amongVeterinary world · 2024Article
- Child Melioidosis Deaths Caused by Burkholderia pseudomallei-Contaminated Borehole Water, Vietnam, 2019.Emerging infectious diseases · 2022Article
- Multiple phylogenetically-diverse, differentially-virulent Burkholderia pseudomallei isolated from a single soil sample collected in Thailand.PLoS neglected tropical diseases · 2022Article
- Multi locus sequence typing of clinical Burkholderia pseudomallei isolates from Malaysia.PLoS neglected tropical diseases · 2020Article
- Burkholderia pseudomallei, the causative agent of melioidosis, is rare but ecologically established and widely dispersed in the environment in Puerto Rico.PLoS neglected tropical diseases · 2019Article
- Prevalence and genetic diversity of Burkholderia pseudomallei isolates in the environment near a patient's residence in Northeast Thailand.PLoS neglected tropical diseases · 2019Article
- Effect of temperature on Burkholderia pseudomallei growth, proteomic changes, motility and resistance to stress environments.Scientific reports · 2018Article
- Multilocus sequence types of clinical Burkholderia pseudomallei isolates from peninsular Malaysia and their associations with disease outcomes.BMC infectious diseases · 2018Article
- Presence ofThe American journal of tropical medicine and hygiene · 2017Article
- Phylogeographic, genomic, and meropenem susceptibility analysis of Burkholderia ubonensis.PLoS neglected tropical diseases · 2017Article
- Burkholderia pseudomallei in a lowland rice paddy: seasonal changes and influence of soil depth and physico-chemical properties.Scientific reports · 2017Article
- Multitarget Quantitative PCR Improves Detection and Predicts Cultivability of the Pathogen Burkholderia pseudomallei.Applied and environmental microbiology · 2017Article
- Structural diversity of Burkholderia pseudomallei lipopolysaccharides affects innate immune signaling.PLoS neglected tropical diseases · 2017Article
- Article
- Validation of a monoclonal antibody-based immunofluorescent assay to detect Burkholderia pseudomallei in blood cultures.Transactions of the Royal Society of Tropical Medicine and Hygiene · 2016Article
- Land use and soil type determine the presence of the pathogen Burkholderia pseudomallei in tropical rivers.Environmental science and pollution research international · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundThe soil-dwelling Gram-negative bacterium Burkholderia pseudomallei is the cause of melioidosis. Extreme structuring of genotype and genotypic frequency has been demonstrated for B. pseudomallei in uncultivated land, but its distribution and genetic diversity in agricultural land where most human infections are probably acquired is not well defined.
methodsFixed-interval soil sampling was performed in a rice paddy in northeast Thailand in which 100 grams of soil was sampled at a depth of 30 cm from 10x10 sampling points each measuring 2.5 m by 2.5 m. Soil was cultured for the presence of B. pseudomallei and genotyping of colonies present on primary culture plates was performed using a combination of pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). PRINCIPAL
findingsB. pseudomallei was cultured from 28/100 samples. Genotyping of 630 primary colonies drawn from 11 sampling points demonstrated 10 PFGE banding pattern types, which on MLST were resolved into 7 sequence types (ST). Overlap of genotypes was observed more often between sampling points that were closely positioned. Two sampling points contained mixed B. pseudomallei genotypes, each with a numerically dominant genotype and one or more additional genotypes present as minority populations.
conclusionsGenetic diversity and structuring of B. pseudomallei exists despite the effects of flooding and the physical and chemical processes associated with farming. These findings form an important baseline for future studies of environmental B. pseudomallei.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.