ArticleGenetics2009
The association of yKu with subtelomeric core X sequences prevents recombination involving telomeric sequences.
Article in Genetics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
- In vivo chromatin organization on native yeast telomeric regions is independent of a cis-telomere loopback conformation.Epigenetics & chromatin · 2020Article
- Histone depletion prevents telomere fusions in pre-senescent cells.PLoS genetics · 2018Article
- Recombination at subtelomeres is regulated by physical distance, double-strand break resection and chromatin status.The EMBO journal · 2017Article
- Contrasting evolutionary genome dynamics between domesticated and wild yeasts.Nature genetics · 2017Article
- Ku Binding on Telomeres Occurs at Sites Distal from the Physical Chromosome Ends.PLoS genetics · 2016Article
- Chromosome-refolding model of mating-type switching in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- The Chromatin and Transcriptional Landscape of Native Saccharomyces cerevisiae Telomeres and Subtelomeric Domains.Genetics · 2015Article
- Genome-wide studies of telomere biology in budding yeast.Microbial cell (Graz, Austria) · 2014Review
- Article
- Epigenetics in Saccharomyces cerevisiae.Cold Spring Harbor perspectives in biology · 2013Review
- Structure and function in the budding yeast nucleus.Genetics · 2012Review
- Everything you ever wanted to know about Saccharomyces cerevisiae telomeres: beginning to end.Genetics · 2012Review
- Ku must load directly onto the chromosome end in order to mediate its telomeric functions.PLoS genetics · 2011Article
- The PIAS homologue Siz2 regulates perinuclear telomere position and telomerase activity in budding yeast.Nature cell biology · 2011Article
- Ku regulates the non-homologous end joining pathway choice of DNA double-strand break repair in human somatic cells.PLoS genetics · 2010Article
- Repressive and non-repressive chromatin at native telomeres in Saccharomyces cerevisiae.Epigenetics & chromatin · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 5 institutions in 3 countries.
Funding
Abstract
The yKu protein of Saccharomyces cerevisiae is important for genome stability by repressing recombination involving telomeric sequences. The mechanism of this repression is not known, but silent heterochromatin such as HML, HMR, and telomeres are compartmentalized at the nuclear periphery and yKu is proposed to interact with these regions and to play a role in telomeric silencing and tethering. We have utilized ChIP on chip, quantitative PCR, and quantitative recombination assays to analyze yKu binding and its effect on genome stability in wild-type and mutant backgrounds. Our data suggest that, although yKu binds to the TG1-3 repeats and other parts of the genome when needed, such as during nonhomologous end-joining, it specifically binds to core X sequences in addition to the mating-type loci, HML and HMR. Association with core X occurred in the absence of Sir proteins, and enhanced binding was observed at silenced ends compared to nonsilenced ends. In contrast, binding to HML and HMR was totally dependent on Sir2-4p and partially dependent on Sir1p with a stronger association at HML in both MATa and MATalpha strains. Using yku80 separation-of-function mutants, we show a direct correlation between core X binding and recombination rate. We believe our findings support our hypothesis that yKu and core X play a pivotal role in maintaining genome stability through nuclear architecture by mediating a defensive fold-back structure at yeast chromosome ends.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.