ArticleGenes & development2009
A regulator from Chlamydia trachomatis modulates the activity of RNA polymerase through direct interaction with the beta subunit and the primary sigma subunit.
Article in Genes & development, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 39 citations in OpenAlex.
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- Coordinated topoisomerase function shapes the fluoroquinolone response ofbioRxiv : the preprint server for biology · 2026Article
- Bacterial two-hybrid systems evolved: innovations for protein-protein interaction research.Journal of bacteriology · 2025Review
- Insights into the association of the Chlamydia trachomatis type III secretion chaperone complex, Scc4:Scc1, from sequential expression in Escherichia coli.Protein expression and purification · 2024Article
- Type III Secretion inMicrobiology and molecular biology reviews : MMBR · 2023Review
- Diversity of σJournal of bacteriology · 2023Article
- Molecular pathogenesis ofFrontiers in cellular and infection microbiology · 2023Review
- A Bacteriophage DNA Mimic Protein Employs a Non-specific Strategy to Inhibit the Bacterial RNA Polymerase.Frontiers in microbiology · 2021Article
- Chain-Selective Isotopic Labeling of the Heterodimeric Type III Secretion Chaperone, Scc4:Scc1, Reveals the Total Structural Rearrangement of theBiomolecules · 2020Article
- Backbone and sidechain resonance assignments and secondary structure of Scc4 from Chlamydia trachomatis.Biomolecular NMR assignments · 2020Article
- Context-Dependent Action of Scc4 Reinforces Control of the Type III Secretion System.Journal of bacteriology · 2020Article
- The σ Subunit-Remodeling Factors: An Emerging Paradigms of Transcription Regulation.Frontiers in microbiology · 2020Review
- Purification of Tag-FreeBiochemistry · 2019Article
- Sequence Determinants Spanning -10 Motif and Spacer Region Implicated in UniqueFrontiers in microbiology · 2019Article
- Characterization of lasR-deficient clinical isolates of Pseudomonas aeruginosa.Scientific reports · 2018Article
- Potency of Solithromycin against Fast- and Slow-Growing Chlamydial Organisms.Antimicrobial agents and chemotherapy · 2018Article
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- Sequence determinants spanning -35 motif and AT-rich spacer region impacting Ehrlichia chaffeensis Sigma 70-dependent promoter activity of two differentially expressed p28 outer membrane protein genes.DNA research : an international journal for rapid publication of reports on genes and genomes · 2016Article
- Quantifying promoter activity during the developmental cycle of Chlamydia trachomatis.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
11 authors at 6 institutions in 3 countries.
Funding
Abstract
The obligate intracellular human pathogen Chlamydia trachomatis undergoes a complex developmental program involving transition between two forms: the infectious elementary body (EB), and the rapidly dividing reticulate body (RB). However, the regulators controlling this development have not been identified. To uncover potential regulators of transcription in C. trachomatis, we screened a C. trachomatis genomic library for sequences encoding proteins that interact with RNA polymerase (RNAP). We report the identification of one such protein, CT663, which interacts with the beta and sigma subunits of RNAP. Specifically, we show that CT663 interacts with the flap domain of the beta subunit (beta-flap) and conserved region 4 of the primary sigma subunit (sigma(66) in C. trachomatis). We find that CT663 inhibits sigma(66)-dependent (but not sigma(28)-dependent) transcription in vitro, and we present evidence that CT663 exerts this effect as a component of the RNAP holoenzyme. The analysis of C. trachomatis-infected cells reveals that CT663 begins to accumulate at the commencement of the RB-to-EB transition. Our findings suggest that CT663 functions as a negative regulator of sigma(66)-dependent transcription, facilitating a global change in gene expression. The strategy used here is generally applicable in cases where genetic tools are unavailable.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.