ArticleMolecular and cellular biology2009
Gene activation by dissociation of an inhibitor from a transcriptional activation domain.
Article in Molecular and cellular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 44 citations in OpenAlex.
- A mechanism of synergistic Mediator recruitment in RNA polymerase II transcription activation revealed by single-molecule fluorescence.Molecular cell · 2025Article
- Transcriptional repressor Gal80 recruits corepressor complex Cyc8-Tup1 to structural genes of the Saccharomyces cerevisiae GAL regulon.Current genetics · 2022Article
- Decoupling transcription factor expression and activity enables dimmer switch gene regulation.Science (New York, N.Y.) · 2021Article
- A double role of the Gal80 N terminus in activation of transcription by Gal4p.Life science alliance · 2020Article
- Live-cell imaging reveals the interplay between transcription factors, nucleosomes, and bursting.The EMBO journal · 2019Article
- Epigenetic memory in gene regulation and immune response.BMB reports · 2019Review
- Assigning function to natural allelic variation via dynamic modeling of gene network induction.Molecular systems biology · 2018Article
- The 9aaTAD Is Exclusive Activation Domain in Gal4.PloS one · 2017Article
- LncRNAs: Bridging environmental sensing and gene expression.RNA biology · 2016Article
- Different Mechanisms Confer Gradual Control and Memory at Nutrient- and Stress-Regulated Genes in Yeast.Molecular and cellular biology · 2015Article
- DNA Topoisomerases Are Required for Preinitiation Complex Assembly during GAL Gene Activation.PloS one · 2015Article
- Sequence context and crosslinking mechanism affect the efficiency of in vivo capture of a protein-protein interaction.Biopolymers · 2014Article
- Approaches to studying subnuclear organization and gene-nuclear pore interactions.Methods in cell biology · 2014Article
- Self-association of the Gal4 inhibitor protein Gal80 is impaired by Gal3: evidence for a new mechanism in the GAL gene switch.Molecular and cellular biology · 2013Article
- Regulations of sugar transporters: insights from yeast.Current genetics · 2013Review
- Fine-tuning multiprotein complexes using small molecules.ACS chemical biology · 2012Review
- Molecular simulation and docking studies of Gal1p and Gal3p proteins in the presence and absence of ligands ATP and galactose: implication for transcriptional activation of GAL genes.Journal of computer-aided molecular design · 2012Article
- Transcription factor binding to a DNA zip code controls interchromosomal clustering at the nuclear periphery.Developmental cell · 2012Article
- Interplay of a ligand sensor and an enzyme in controlling expression of the Saccharomyces cerevisiae GAL genes.Eukaryotic cell · 2012Article
- The Gal3p transducer of the GAL regulon interacts with the Gal80p repressor in its ligand-induced closed conformation.Genes & development · 2012Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Gal4 is a prototypical eukaryotic transcriptional activator whose recruitment function is inhibited in the absence of galactose by the Gal80 protein through masking of its transcriptional activation domain (AD). A long-standing nondissociation model posits that galactose-activated Gal3 interacts with Gal4-bound Gal80 at the promoter, yielding a tripartite Gal3-Gal80-Gal4 complex with altered Gal80-Gal4 conformation to enable Gal4 AD activity. Some recent data challenge this model, whereas other recent data support the model. To address this controversy, we imaged fluorescent-protein-tagged Gal80, Gal4, and Gal3 in live cells containing a novel GAL gene array. We find that Gal80 rapidly dissociates from Gal4 in response to galactose. Importantly, this dissociation is Gal3 dependent and concurrent with Gal4-activated GAL gene expression. When galactose-triggered dissociation is followed by galactose depletion, preexisting Gal80 reassociates with Gal4, indicating that sequestration of Gal80 by Gal3 contributes to the observed Gal80-Gal4 dissociation. Moreover, the ratio of nuclear Gal80 to cytoplasmic Gal80 decreases in response to Gal80-Gal3 interaction. Taken together, these and other results provide strong support for a GAL gene switch model wherein Gal80 rapidly dissociates from Gal4 through a mechanism that involves sequestration of Gal80 by galactose-activated Gal3.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.