ReviewPhilosophical transactions of the Royal Society of London. Series B, Biological sciences2009
Wide-field and two-photon imaging of brain activity with voltage- and calcium-sensitive dyes.
Review in Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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Who cites it
33 citing papers in PubMed, 82 citations in OpenAlex.
- Beyond Membrane Potential: Exploiting Signal Complexity in Genetically Encoded Voltage Indicators.Sensors (Basel, Switzerland) · 2026Article
- Noise Sources and Strategies for Signal Quality Improvement in Biological Imaging: A Review Focused on Calcium and Cell Membrane Voltage Imaging.Biosensors · 2026Review
- Primary Cell Cultures in Neurobiology: Optimized Protocol for Culture of Mouse Fetal Hindbrain Neurons.Cells · 2025Article
- Spatio-temporal segregation between sensory relay and swallowing pre-motor population activities by optical imaging in the rat nucleus of the solitary tract.Pflugers Archiv : European journal of physiology · 2025Article
- Neuronal Imaging at 8-Bit Depth to Combine High Spatial and High Temporal Resolution With Acquisition Rates Up To 40 kHz.Journal of biophotonics · 2025Article
- Discovering Microcircuit Secrets With Multi-Spot Imaging and Electrophysiological Recordings: The Example of Cerebellar Network Dynamics.Frontiers in cellular neuroscience · 2022Review
- Voltage Imaging with a NIR-Absorbing Phosphine Oxide Rhodamine Voltage Reporter.Journal of the American Chemical Society · 2021Article
- Article
- Optical interrogation of neuronal circuitry in zebrafish using genetically encoded voltage indicators.Scientific reports · 2018Article
- Photoacoustic imaging of voltage responses beyond the optical diffusion limit.Scientific reports · 2017Article
- OCT intensity and phase fluctuations correlated with activity-dependent neuronal calcium dynamics in theBiomedical optics express · 2017Article
- Synthesis of Near-Infrared Fluorescent Two-Photon-Absorbing Fluorenyl Benzothiadiazole and Benzoselenadiazole Derivatives.ACS omega · 2016Article
- Effects of GABAA kinetics on cortical population activity: computational studies and physiological confirmations.Journal of neurophysiology · 2016Article
- Capitalizing on Directed Evolution and Rational Protein Engineering to Expand the Neuroscientist's Imaging Toolbox.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Review
- Comparison of glomerular activity patterns by fMRI and wide-field calcium imaging: Implications for principles underlying odor mapping.NeuroImage · 2016Article
- Optical coherence tomography for cross-sectional imaging of neural activity.Neurophotonics · 2015Article
- 3D in vitro modeling of the central nervous system.Progress in neurobiology · 2015Review
- Mesoscale transcranial spontaneous activity mapping in GCaMP3 transgenic mice reveals extensive reciprocal connections between areas of somatomotor cortex.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Connectomics and epilepsy.Current opinion in neurology · 2013Article
- Multi-electrode array technologies for neuroscience and cardiology.Nature nanotechnology · 2013Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
This review presents three examples of using voltage- or calcium-sensitive dyes to image the activity of the brain. Our aim is to discuss the advantages and disadvantages of each method with particular reference to its application to the study of the brainstem. Two of the examples use wide-field (one-photon) imaging; the third uses two-photon scanning microscopy. Because the measurements have limited signal-to-noise ratio, the paper also discusses the methodological aspects that are critical for optimizing the signal. The three examples are the following. (i) An intracellularly injected voltage-sensitive dye was used to monitor membrane potential in the dendrites of neurons in in vitro preparations. These experiments were directed at understanding how individual neurons convert complex synaptic inputs into the output spike train. (ii) An extracellular, bath application of a voltage-sensitive dye was used to monitor population signals from different parts of the dorsal brainstem. We describe recordings made during respiratory activity. The population signals indicated four different regions with distinct activity correlated with inspiration. (iii) Calcium-sensitive dyes can be used to label many individual cells in the mammalian brain. This approach, combined with two-photon microscopy, made it possible to follow the spike activity in an in vitro brainstem preparation during fictive respiratory rhythms. The organic voltage- and ion-sensitive dyes used today indiscriminatively stain all of the cell types in the preparation. A major effort is underway to develop fluorescent protein sensors of activity for selectively staining individual cell types.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.