ArticleBiophysical journal2009
Impact of emission anisotropy on fluorescence spectroscopy and FRET distance measurements.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins.Nature methods · 2023Article
- Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features.Journal of visualized experiments : JoVE · 2022Article
- FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices.eLife · 2021Review
- Mismatch Recognition byInternational journal of molecular sciences · 2019Article
- MutSγ-Induced DNA Conformational Changes Provide Insights into Its Role in Meiotic Recombination.Biophysical journal · 2018Article
- Directional Photonic Wire Mediated by Homo-Förster Resonance Energy Transfer on a DNA Origami Platform.ACS nano · 2017Article
- Alignment of the protein substrate hairpin along the SecA two-helix finger primes protein transport inProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Fluorescence Resonance Energy Transfer Characterization of DNA Wrapping in Closed and Open Escherichia coli RNA Polymerase-λP(R) Promoter Complexes.Biochemistry · 2016Article
- Nucleoprotein architectures regulating the directionality of viral integration and excision.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Contribution of fluorophore dynamics and solvation to resonant energy transfer in protein-DNA complexes: a molecular-dynamics study.Biophysical journal · 2014Article
- Defining the solution state dimer structure of Escherichia coli SecA using Förster resonance energy transfer.Biochemistry · 2013Article
- Supertertiary structure of the synaptic MAGuK scaffold proteins is conserved.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Measurements of DNA-loop formation via Cre-mediated recombination.Nucleic acids research · 2012Article
- Effects of Hofmeister ions on the α-helical structure of proteins.Biophysical journal · 2012Article
- DNA requirements for assembly and stability of HIV-1 intasomes.Protein science : a publication of the Protein Society · 2012Article
- Three-dimensional molecular modeling with single molecule FRET.Journal of structural biology · 2011Review
- Stoichiometric incorporation of base substitutions at specific sites in supercoiled DNA and supercoiled recombination intermediates.Nucleic acids research · 2010Article
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Authors and funding
3 authors.
Funding
Abstract
The objective of this report is to provide a practical and improved method for estimating Förster resonance energy transfer distance measurement error due to unknown angles in the dipole orientation factor based on emission anisotropy measurements. We improve on the method of Dale et al. (1979), which has minor mistakes and is frequently interpreted in overly optimistic ways in the literature. To facilitate proper fluorescence intensity measurements, we also evaluated instrument parameters that could impact the measurement. The apparent fluorescence intensity of isotropic samples depends on the sample emission anisotropy, fluorometer geometry, and optical apertures. We separate parameters of the sample, and those of the cylindrically symmetric illumination source and detector in the equations describing results of unpolarized and polarized fluorescence intensity measurements. This approach greatly simplifies calculations compared with the more universal method of Axelrod (1989). We provide a full computational method for calculating the Förster resonance energy transfer distance error and present a graph describing distance error in the simplest case.
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