ArticleBiophysical journal2009
Single-molecule study of metalloregulator CueR-DNA interactions using engineered Holliday junctions.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- TheGenes · 2026Article
- The Copper Efflux Regulator (CueR).Sub-cellular biochemistry · 2024Review
- Metabolic and Oxidative Stress Effects on the Spectroelectrochemical Behavior of SingleChemical & biomedical imaging · 2023Article
- The use of EPR spectroscopy to study transcription mechanisms.Biophysical reviews · 2022Review
- Allostery-driven changes in dynamics regulate the activation of bacterial copper transcription factor.Protein science : a publication of the Protein Society · 2022Article
- Copper Homeostatic Mechanisms and Their Role in the Virulence of Escherichia coli and Salmonella enterica.EcoSal Plus · 2021Review
- A study on the secondary structure of the metalloregulatory protein CueR: effect of pH, metal ions and DNA.European biophysics journal : EBJ · 2021Article
- Facilitated Unbinding via Multivalency-Enabled Ternary Complexes: New Paradigm for Protein-DNA Interactions.Accounts of chemical research · 2018Review
- TRANSCRIPTION. Allosteric transcriptional regulation via changes in the overall topology of the core promoter.Science (New York, N.Y.) · 2015Article
- Concentration- and chromosome-organization-dependent regulator unbinding from DNA for transcription regulation in living cells.Nature communications · 2015Article
- Pathogenic adaptations to host-derived antibacterial copper.Frontiers in cellular and infection microbiology · 2014Review
- Single-molecule dynamics and mechanisms of metalloregulators and metallochaperones.Biochemistry · 2013Review
- Protein signatures that promote operator selectivity among paralog MerR monovalent metal ion regulators.The Journal of biological chemistry · 2013Article
- Direct substitution and assisted dissociation pathways for turning off transcription by a MerR-family metalloregulator.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Tackling metal regulation and transport at the single-molecule level.Natural product reports · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
To maintain normal metal metabolism, bacteria use metal-sensing metalloregulators to control transcription of metal resistance genes. Depending on their metal-binding states, the MerR-family metalloregulators change their interactions with DNA to suppress or activate transcription. To understand their functions fundamentally, we study how CueR, a Cu(1+)-responsive MerR-family metalloregulator, interacts with DNA, using an engineered DNA Holliday junction (HJ) as a protein-DNA interaction reporter in single-molecule fluorescence resonance energy transfer measurements. By analyzing the single-molecule structural dynamics of the engineered HJ in the presence of various concentrations of both apo- and holo-CueR, we show how CueR interacts with the two conformers of the engineered HJ, forming variable protein-DNA complexes at different protein concentrations and changing the HJ structures. We also show how apo- and holo-CueR differ in their interactions with DNA, and discuss their similarities and differences with other MerR-family metalloregulators. The surprising finding that holo-CueR binds more strongly to DNA than to apo-CueR suggests functional differences among MerR-family metalloregulators, in particular in their mechanisms of switching off gene transcription after activation. The study also corroborates the general applicability of engineered HJs as single-molecule reporters for protein-DNA interactions, which are fundamental processes in gene replication, transcription, recombination, and regulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.