ArticleBMC biology2009
TBP2 is a substitute for TBP in Xenopus oocyte transcription.
Article in BMC biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 35 citations in OpenAlex.
- TBP facilitates RNA Polymerase I transcription following mitosis.RNA biology · 2024Article
- Functional implications of paralog genes in polyglutamine spinocerebellar ataxias.Human genetics · 2023Review
- Article
- What defines the maternal transcriptome?Biochemical Society transactions · 2021Review
- TBPL2/TFIIA complex establishes the maternal transcriptome through oocyte-specific promoter usage.Nature communications · 2020Article
- TATA box-binding protein-related factor 3 drives the mesendoderm specification of human embryonic stem cells by globally interacting with the TATA box of key mesendodermal genes.Stem cell research & therapy · 2020Article
- Activation of a T-box-Otx2-Gsc gene network independent of TBP and TBP-related factors.Development (Cambridge, England) · 2016Article
- Hierarchical molecular events driven by oocyte-specific factors lead to rapid and extensive reprogramming.Molecular cell · 2014Article
- Article
- Article
- The epigenome in early vertebrate development.Genesis (New York, N.Y. : 2000) · 2012Review
- TBP-related factors: a paradigm of diversity in transcription initiation.Cell & bioscience · 2011Article
- General transcription factors and subunits of RNA polymerase III: Paralogs for promoter- and cell type-specific transcription in multicellular eukaryotes.Transcription · 2010Article
- Unexpected roles for core promoter recognition factors in cell-type-specific transcription and gene regulation.Nature reviews. Genetics · 2010Review
- A paired-end sequencing strategy to map the complex landscape of transcription initiation.Nature methods · 2010Article
- TBP2 is a general transcription factor specialized for female germ cells.Journal of biology · 2009Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTATA-box-binding protein 2 (TBP2/TRF3) is a vertebrate-specific paralog of TBP that shares with TBP a highly conserved carboxy-terminal domain and the ability to bind the TATA box. TBP2 is highly expressed in oocytes whereas TBP is more abundant in embryos.
resultsWe find that TBP2 is proteolytically degraded upon meiotic maturation; after germinal vesicle breakdown relatively low levels of TBP2 expression persist. Furthermore, TBP2 localizes to the transcriptionally active loops of lampbrush chromosomes and is recruited to a number of injected promoters in oocyte nuclei. Using an altered binding specificity mutant reporter system we show that TBP2 promotes RNA polymerase II transcription in vivo. Intriguingly, TBP, which in oocytes is undetectable at the protein level, can functionally replace TBP2 when ectopically expressed in oocytes, showing that switching of initiation factors can be driven by changes in their expression. Proteolytic degradation of TBP2 is not required for repression of transcription during meiotic maturation, suggesting a redundant role in this repression or a role in initiation factor switching between oocytes and embryos.
conclusionThe expression and transcriptional activity of TBP2 in oocytes show that TBP2 is the predominant initiation factor in oocytes, which is substituted by TBP on a subset of promoters in embryos as a result of proteolytic degradation of TBP2 during meiotic maturation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.