ArticlePlant methods2009
3D fluorescent in situ hybridization using Arabidopsis leaf cryosections and isolated nuclei.
Article in Plant methods, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- HOMEODOMAIN-LIKE shapes chromatin architecture and rewires leaf epidermal patterns.Nature communications · 2026Article
- Best practices in plant fluorescence imaging and reporting: A primer.The Plant cell · 2025Review
- Abundant clock proteins point to missing molecular regulation in the plant circadian clock.Molecular systems biology · 2025Article
- Cell geometry regulates tissue fracture.Nature communications · 2023Article
- Opportunities and challenges in the application of single-cell and spatial transcriptomics in plants.Frontiers in plant science · 2023Review
- Localization of the Swainsonine-Producing Chaetothyriales Symbiont in the Seed and Shoot Apical Meristem in Its HostMicroorganisms · 2022Article
- MicroRNA biogenesis and activity in plant cell dedifferentiation stimulated by cell wall removal.BMC plant biology · 2022Article
- Visualization of Protein Coding, Long Noncoding, and Nuclear RNAs by Fluorescence in Situ Hybridization in Sections of Shoot Apical Meristems and Developing Flowers.Plant physiology · 2020Article
- Salt stress vs. salt shock - the case of sugar beet and its halophytic ancestor.BMC plant biology · 2019Article
- Auxin decreases chromatin accessibility through the TIR1/AFBs auxin signaling pathway in proliferative cells.Scientific reports · 2018Article
- Visualization of specific repetitive genomic sequences with fluorescent TALEs in Arabidopsis thaliana.Journal of experimental botany · 2016Article
- Molecular characterization of flavanone 3-hydroxylase gene and flavonoid accumulation in two chemotyped safflower lines in response to methyl jasmonate stimulation.BMC plant biology · 2016Article
- Spatial relationship between chromosomal domains in diploid and autotetraploid Arabidopsis thaliana nuclei.Nucleus (Austin, Tex.) · 2016Article
- Article
- Matrix-assisted laser desorption/ionisation mass spectrometry imaging and its development for plant protein imaging.Plant methods · 2011Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFluorescent hybridization techniques are widely used to study the functional organization of different compartments within the mammalian nucleus. However, few examples of such studies are known in the plant kingdom. Indeed, preservation of nuclei 3D structure, which is required for nuclear organization studies, is difficult to fulfill.
resultsWe report a rapid protocol for fluorescent in situ hybridization (FISH) performed on 3D isolated nuclei and thin cryosectioned leaves of Arabidopsis thaliana. The use of direct labeling minimized treatment steps, shortening the overall procedure. Using image analysis, we measured different parameters related to nucleus morphology and overall 3D structure.
conclusionOur work describes a 3D-FISH protocol that preserves the 3D structure of Arabidopsis interphase nuclei. Moreover, we report for the first time FISH using cryosections of Arabidopsis leaves. This protocol is a valuable tool to investigate nuclear architecture and chromatin organization.
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