ArticleMolecular microbiology2009
Genome-wide screens: novel mechanisms in colicin import and cytotoxicity.
Article in Molecular microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 43 citations in OpenAlex.
- High-Throughput Screening Method UsingMicroorganisms · 2024Article
- Can antimicrobial blue light contribute to resistance development? Genome-wide analysis revealed aBL-protective genes inMicrobiology spectrum · 2024Article
- O antigen biogenesis sensitises Escherichia coli K-12 to bile salts, providing a plausible explanation for its evolutionary loss.PLoS genetics · 2023Article
- Bifurcated binding of the OmpF receptor underpins import of the bacteriocin colicin N intoThe Journal of biological chemistry · 2020Article
- Novel Chromosomal Mutations Responsible for Fosfomycin Resistance inFrontiers in microbiology · 2020Article
- Pleiotropic effects of rfa-gene mutations on Escherichia coli envelope properties.Scientific reports · 2019Article
- O-Antigen-Dependent Colicin Insensitivity of Uropathogenic Escherichia coli.Journal of bacteriology · 2019Article
- The Colicin E1 TolC Box: Identification of a Domain Required for Colicin E1 Cytotoxicity and TolC Binding.Journal of bacteriology · 2017Article
- The Colicin E1 TolC-Binding Conformer: Pillar or Pore Function of TolC in Colicin Import?Biochemistry · 2016Article
- Contact-dependent growth inhibition toxins exploit multiple independent cell-entry pathways.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Antibacterial toxin colicin N and phage protein G3p compete with TolB for a binding site on TolA.Microbiology (Reading, England) · 2015Article
- A new biofilm-associated colicin with increased efficiency against biofilm bacteria.The ISME journal · 2014Article
- Daring to be different: colicin N finds another way.Molecular microbiology · 2014Article
- The antibacterial toxin colicin N binds to the inner core of lipopolysaccharide and close to its translocator protein.Molecular microbiology · 2014Article
- The unstructured domain of colicin N kills Escherichia coli.Molecular microbiology · 2013Article
- Genome-wide screening with hydroxyurea reveals a link between nonessential ribosomal proteins and reactive oxygen species production.Journal of bacteriology · 2013Article
- Mobility of BtuB and OmpF in the Escherichia coli outer membrane: implications for dynamic formation of a translocon complex.Biophysical journal · 2010Article
- YieJ (CbrC) mediates CreBC-dependent colicin E2 tolerance in Escherichia coli.Journal of bacteriology · 2010Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Only two new genes (fkpA and lepB) have been identified to be required for colicin cytotoxicity in the last 25 years. Genome-wide screening using the 'Keio collection' to test sensitivity to colicins (col) A, B, D, E1, E2, E3, E7 and N from groups A and B, allowed identification of novel genes affecting cytotoxicity and provided new information on mechanisms of action. The requirement of lipopolysaccharide for colN cytotoxicity resides specifically in the lipopolysaccharide inner-core and first glucose. ColA cytotoxicity is dependent on gmhB and rffT genes, which function in the biosynthesis of lipopolysaccharide and enterobacterial common antigen. Of the tol genes that function in the cytoplasmic membrane translocon, colE1 requires tolA and tolR but not tolQ for activity. Peptidoglycan-associated lipoprotein, which interacts with the Tol network, is not required for cytotoxicity of group A colicins. Except for TolQRA, no cytoplasmic membrane protein is essential for cytotoxicity of group A colicins, implying that TolQRA provides the sole pathway for their insertion into/through the cytoplasmic membrane. The periplasmic protease that cleaves between the receptor and catalytic domains of colE7 was not identified, implying either that the responsible gene is essential for cell viability, or that more than one gene product has the necessary proteolysis function.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.