ArticleCurrent genetics2009
Endochitinase CHI2 of the biocontrol fungus Metarhizium anisopliae affects its virulence toward the cotton stainer bug Dysdercus peruvianus.
Article in Current genetics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 85 citations in OpenAlex.
- The role of fungal chitinases in the tripartite interactions among insects, plants and entomopathogenic fungi.Pest management science · 2026Review
- The chitinolytic enzymes from the entomopathogenic fungusFrontiers in fungal biology · 2026Review
- Genome Sequence, Comparative Genome Analysis, and Expression Profiling of the Chitinase GH18 Gene Family inInternational journal of molecular sciences · 2025Article
- Microbial chitinases and their relevance in various industries.Folia microbiologica · 2023Review
- Unraveling the Secrets of a Double-Life Fungus by Genomics:Journal of fungi (Basel, Switzerland) · 2023Article
- Several Metarhizium Species Produce Ergot Alkaloids in a Condition-Specific Manner.Applied and environmental microbiology · 2020Article
- Article
- A Plumieridine-Rich Fraction FromFrontiers in microbiology · 2020Article
- Chitinase genes fromRoyal Society open science · 2019Article
- Secretome Profiling Reveals Virulence-Associated Proteins ofBiomolecules · 2019Article
- Comparative genomic analyses reveal the features for adaptation to nematodes in fungi.DNA research : an international journal for rapid publication of reports on genes and genomes · 2018Article
- Mode of Infection of Metarhizium spp. Fungus and Their Potential as Biological Control Agents.Journal of fungi (Basel, Switzerland) · 2017Review
- Ovicidal effect of chitinase and protease enzymes produced by soil fungi on the camel tickJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2017Article
- Comparative genome analysis of entomopathogenic fungi reveals a complex set of secreted proteins.BMC genomics · 2014Article
- Article
- Neutral lipid composition changes in the fat bodies of engorged females Rhipicephalus microplus ticks in response to fungal infections.Parasitology research · 2013Article
- Use of Metarhizium anisopliae chitinase genes for genotyping and virulence characterization.BioMed research international · 2013Article
- Fungal chitinases: diversity, mechanistic properties and biotechnological potential.Applied microbiology and biotechnology · 2012Review
- Cloning and expression analysis of the chitinase gene Ifu-chit2 from Isaria fumosorosea.Genetics and molecular biologyArticle
- Molecular evolution and transcriptional profile of GH3 and GH20 β-N-acetylglucosaminidases in the entomopathogenic fungus Metarhizium anisopliae.Genetics and molecular biologyArticle
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chitinases have been implicated in fungal cell wall remodeling and play a role in exogenous chitin degradation for nutrition and competition. Due to the diversity of these enzymes, assigning particular functions to each chitinase is still ongoing. The entomopathogenic fungus Metarhizium anisopliae produces several chitinases, and here, we evaluate whether endochitinase CHI2 is involved in the pathogenicity of this fungus. We constructed strains either overexpressing or lacking the CHI2 chitinase. These constructs were validated by Southern, Northern and Western blot analysis, and chitinase production. To access the effects of CHI2 chitinase in virulence, the cotton stainer bug Dysdercus peruvianus was used as a host. CHI2 overexpression constructs showed higher efficiency in host killing suggesting that the production of this chitinase by a constitutive promoter reduces the time necessary to kill the insect. More significantly, the knock out constructs showed decreased virulence to the insects as compared to the wild type strain. The lack of this single CHI2 chitinase diminished fungal infection efficiency, but not any other detectable trait, showing that the M. anisopliae family 18, subgroup B endochitinase CHI2 plays a role in insect infection.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.