ArticlePLoS pathogens2009
Comprehensive identification of Salmonella enterica serovar typhimurium genes required for infection of BALB/c mice.
Article in PLoS pathogens, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
- aurora: a machine learning gwas tool for analyzing microbial habitat adaptation.Genome biology · 2025Article
- Optimizing phage-based mutant recovery and minimizing heat effect in the construction of transposon libraries in Staphylococcus aureus.Scientific reports · 2024Article
- Determinants of bacterial survival and proliferation in blood.FEMS microbiology reviews · 2024Review
- A tRNA modifying enzyme as a tunable regulatory nexus for bacterial stress responses and virulence.Nucleic acids research · 2022Article
- Genome archaeology of two laboratory Salmonella enterica enterica sv Typhimurium.G3 (Bethesda, Md.) · 2021Article
- Salmonella enterica Serovars Dublin and Enteritidis Comparative Proteomics Reveals Differential Expression of Proteins Involved in Stress Resistance, Virulence, and Anaerobic Metabolism.Infection and immunity · 2021Article
- Article
- Metabolic and genetic basis for auxotrophies in Gram-negative species.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- The Small RNA PinT Contributes to PhoP-Mediated Regulation of theJournal of bacteriology · 2019Article
- The Transcription Factor ArcA ModulatesFrontiers in microbiology · 2019Article
- Genome-scale metabolic reconstructions of multiple Salmonella strains reveal serovar-specific metabolic traits.Nature communications · 2018Article
- Identification of a new alanine racemase inGut pathogens · 2018Article
- RNA-seq and Tn-seq reveal fitness determinants of vancomycin-resistant Enterococcus faecium during growth in human serum.BMC genomics · 2017Article
- Salmonella Persistence in Tomatoes Requires a Distinct Set of Metabolic Functions Identified by Transposon Insertion Sequencing.Applied and environmental microbiology · 2017Article
- Centrality in the host-pathogen interactome is associated with pathogen fitness during infection.Nature communications · 2017Article
- Genes Required for the Fitness of Salmonella enterica Serovar Typhimurium during Infection of Immunodeficient gp91-/- phox Mice.Infection and immunity · 2016Article
- Analysis of Two Complementary Single-Gene Deletion Mutant Libraries of Salmonella Typhimurium in Intraperitoneal Infection of BALB/c Mice.Frontiers in microbiology · 2015Article
- Regulation of fucose and 1,2-propanediol utilization by Salmonella enterica serovar Typhimurium.Frontiers in microbiology · 2015Article
- Fructose-asparagine is a primary nutrient during growth of Salmonella in the inflamed intestine.PLoS pathogens · 2014Article
- Identification of metabolic pathways essential for fitness of Salmonella Typhimurium in vivo.PloS one · 2014Article
Corrections and comments
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Authors and funding
13 authors.
Funding
Abstract
Genes required for infection of mice by Salmonella Typhimurium can be identified by the interrogation of random transposon mutant libraries for mutants that cannot survive in vivo. Inactivation of such genes produces attenuated S. Typhimurium strains that have potential for use as live attenuated vaccines. A quantitative screen, Transposon Mediated Differential Hybridisation (TMDH), has been developed that identifies those members of a large library of transposon mutants that are attenuated. TMDH employs custom transposons with outward-facing T7 and SP6 promoters. Fluorescently-labelled transcripts from the promoters are hybridised to whole-genome tiling microarrays, to allow the position of the transposon insertions to be determined. Comparison of microarray data from the mutant library grown in vitro (input) with equivalent data produced after passage of the library through mice (output) enables an attenuation score to be determined for each transposon mutant. These scores are significantly correlated with bacterial counts obtained during infection of mice using mutants with individual defined deletions of the same genes. Defined deletion mutants of several novel targets identified in the TMDH screen are effective live vaccines.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.