ArticlePloS one2009
Community analysis of chronic wound bacteria using 16S rRNA gene-based pyrosequencing: impact of diabetes and antibiotics on chronic wound microbiota.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 109 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
109 citing papers in PubMed, 1 synthesis or guideline pooled it, 233 citations in OpenAlex.
- A systematic literature review of the human skin microbiome as biomarker for dermatological drug development.British journal of clinical pharmacology · 2018Pooled it
- Examining the relationship between maternal body size, gestational glucose tolerance status, mode of delivery and ethnicity on human milk microbiota at three months post-partum.BMC microbiology · 2020Trial
- Article
- Epidemiological Study of the Relationship Between Antimicrobial Resistance Genes and Biofilm-Forming Capacity in Pathogens Causing Chronic Wound Infections.Microorganisms · 2026Article
- Article
- Postoperative wound microbiota in diabetic patients with perianal abscess: a 16s rRNA gene sequencing study.BMC microbiology · 2025Article
- Review
- Integration of localized microbiome, metabolome, and clinical datasets predicts healing in chronic wounds among veterans.bioRxiv : the preprint server for biology · 2025Article
- The wound microbiome associated with deep sternal wound infection: a scoping review.Journal of thoracic disease · 2025Review
- The Impact of the Skin Microbiome and Oxidative Stress on the Initiation and Development of Cutaneous Chronic Wounds.Antioxidants (Basel, Switzerland) · 2025Review
- Cold atmospheric plasma and skin wound healing: influence on microbial diversity and composition.BMC microbiology · 2025Article
- Development of a clinical metagenomics workflow for the diagnosis of wound infections.BMC medical genomics · 2024Article
- Biofilm-mediated infections by multidrug-resistant microbes: a comprehensive exploration and forward perspectives.Archives of microbiology · 2024Review
- Review
- Article
- Contribution to the characterization of the seed endophyte microbiome ofFrontiers in microbiology · 2024Article
- Next Steps: Studying Diabetic Foot Infections with Next-Generation Molecular Assays.Current infectious disease reports · 2023Article
- Gut Microbiota Patterns in Patients with Non-Alcoholic Fatty Liver Disease: A Comprehensive Assessment Using Three Analysis Methods.International journal of molecular sciences · 2023Article
- Multi-Instrumental Analysis Toward Exploring the Diabetic Foot Infection Microbiota.Current microbiology · 2023Article
- The Wound Microbiome.Cold Spring Harbor perspectives in biology · 2023Review
49 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBacterial colonization is hypothesized to play a pathogenic role in the non-healing state of chronic wounds. We characterized wound bacteria from a cohort of chronic wound patients using a 16S rRNA gene-based pyrosequencing approach and assessed the impact of diabetes and antibiotics on chronic wound microbiota. METHODOLOGY/PRINCIPAL
findingsWe prospectively enrolled 24 patients at a referral wound center in Baltimore, MD; sampled patients' wounds by curette; cultured samples under aerobic and anaerobic conditions; and pyrosequenced the 16S rRNA V3 hypervariable region. The 16S rRNA gene-based analyses revealed an average of 10 different bacterial families in wounds--approximately 4 times more than estimated by culture-based analyses. Fastidious anaerobic bacteria belonging to the Clostridiales family XI were among the most prevalent bacteria identified exclusively by 16S rRNA gene-based analyses. Community-scale analyses showed that wound microbiota from antibiotic treated patients were significantly different from untreated patients (p = 0.007) and were characterized by increased Pseudomonadaceae abundance. These analyses also revealed that antibiotic use was associated with decreased Streptococcaceae among diabetics and that Streptococcaceae was more abundant among diabetics as compared to non-diabetics. CONCLUSIONS/SIGNIFICANCE: The 16S rRNA gene-based analyses revealed complex bacterial communities including anaerobic bacteria that may play causative roles in the non-healing state of some chronic wounds. Our data suggest that antimicrobial therapy alters community structure--reducing some bacteria while selecting for others.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.