ArticlePloS one2009
Host responses to intestinal microbial antigens in gluten-sensitive mice.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03664531 (Crossover Trial of Purified Gluten and Whole Wheat), which is not on this map. Cited by 30 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Crossover Trial of Purified Gluten and Whole Wheat (Gluten With Amylase-trypsin Inhibitors) as Triggers of Gastrointestinal Symptoms in Patients With Irritable Bowel Syndrome
Who cites it
30 citing papers in PubMed, 2 syntheses or guidelines pooled it, 78 citations in OpenAlex.
- Limited support for a direct connection between prebiotics and intestinal permeability - a systematic review.Glycoconjugate journal · 2024Pooled it
- Guideline
- Non-Celiac Gluten Sensitivity Has Narrowed the Spectrum of Irritable Bowel Syndrome: A Double-Blind Randomized Placebo-Controlled Trial.Nutrients · 2015Trial
- Bacteria: Potential Make-or-Break Determinants of Celiac Disease.International journal of molecular sciences · 2024Review
- Gut barrier-microbiota imbalances in early life lead to higher sensitivity to inflammation in a murine model of C-section delivery.Microbiome · 2023Article
- Paracellular permeability and tight junction regulation in gut health and disease.Nature reviews. Gastroenterology & hepatology · 2023Review
- The Infant-DerivedMicroorganisms · 2020Article
- Motility Disorders in Celiac Disease and Non-Celiac Gluten Sensitivity: The Impact of a Gluten-Free Diet.Nutrients · 2018Review
- The Role of Bacteria, Probiotics and Diet in Irritable Bowel Syndrome.Foods (Basel, Switzerland) · 2018Review
- The Overlapping Area of Non-Celiac Gluten Sensitivity (NCGS) and Wheat-Sensitive Irritable Bowel Syndrome (IBS): An Update.Nutrients · 2017Review
- The intestinal epithelial barrier: a therapeutic target?Nature reviews. Gastroenterology & hepatology · 2017Review
- Long-lived plasma cells are generated in mucosal immune responses and contribute to the bone marrow plasma cell pool in mice.Mucosal immunology · 2016Article
- Role of the gastrointestinal ecosystem in the development of type 1 diabetes.Pediatric diabetes · 2015Review
- Faecalibacterium prausnitzii prevents physiological damages in a chronic low-grade inflammation murine model.BMC microbiology · 2015Article
- Article
- The effect of Lactobacillus rhamnosus hsryfm 1301 on the intestinal microbiota of a hyperlipidemic rat model.BMC complementary and alternative medicine · 2014Article
- Novel role of the serine protease inhibitor elafin in gluten-related disorders.The American journal of gastroenterology · 2014Article
- Article
- Intraluminal administration of poly I:C causes an enteropathy that is exacerbated by administration of oral dietary antigen.PloS one · 2014Article
- Pathogen-specific risk of celiac disease following bacterial causes of foodborne illness: a retrospective cohort study.Digestive diseases and sciences · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
Abstract
BACKGROUND AND
aimsExcessive uptake of commensal bacterial antigens through a permeable intestinal barrier may influence host responses to specific antigen in a genetically predisposed host. The aim of this study was to investigate whether intestinal barrier dysfunction induced by indomethacin treatment affects the host response to intestinal microbiota in gluten-sensitized HLA-DQ8/HCD4 mice. METHODOLOGY/PRINCIPAL
findingsHLA-DQ8/HCD4 mice were sensitized with gluten, and gavaged with indomethacin plus gluten. Intestinal permeability was assessed by Ussing chamber; epithelial cell (EC) ultra-structure by electron microscopy; RNA expression of genes coding for junctional proteins by Q-real-time PCR; immune response by in-vitro antigen-specific T-cell proliferation and cytokine analysis by cytometric bead array; intestinal microbiota by fluorescence in situ hybridization and analysis of systemic antibodies against intestinal microbiota by surface staining of live bacteria with serum followed by FACS analysis. Indomethacin led to a more pronounced increase in intestinal permeability in gluten-sensitized mice. These changes were accompanied by severe EC damage, decreased E-cadherin RNA level, elevated IFN-gamma in splenocyte culture supernatant, and production of significant IgM antibody against intestinal microbiota.
conclusionIndomethacin potentiates barrier dysfunction and EC injury induced by gluten, affects systemic IFN-gamma production and the host response to intestinal microbiota antigens in HLA-DQ8/HCD4 mice. The results suggest that environmental factors that alter the intestinal barrier may predispose individuals to an increased susceptibility to gluten through a bystander immune activation to intestinal microbiota.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.