ArticleThe Journal of biological chemistry2009
Novel role of nucleostemin in the maintenance of nucleolar architecture and integrity of small nucleolar ribonucleoproteins and the telomerase complex.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 43 citations in OpenAlex.
- The Relevance of Telomerase and Telomere-Associated Proteins in B-Acute Lymphoblastic Leukemia.Genes · 2023Review
- Intergenic Interactions ofLife (Basel, Switzerland) · 2023Article
- Review
- Common variants in the GNL3 contribute to the increasing risk of knee osteoarthritis in Han Chinese population.Scientific reports · 2018Article
- Article
- Cellular and Molecular Preconditions for Retinal Pigment Epithelium (RPE) Natural Reprogramming during Retinal Regeneration in Urodela.Biomedicines · 2016Review
- Article
- Stressing on the nucleolus in cardiovascular disease.Biochimica et biophysica acta · 2014Review
- Nucleostemin and GNL3L exercise distinct functions in genome protection and ribosome synthesis, respectively.Journal of cell science · 2014Article
- Allelic expression analysis of the osteoarthritis susceptibility locus that maps to chromosome 3p21 reveals cis-acting eQTLs at GNL3 and SPCS1.BMC medical genetics · 2014Article
- Dyskerin localizes to the mitotic apparatus and is required for orderly mitosis in human cells.PloS one · 2013Article
- Identification of novel markers that demarcate the nucleolus during severe stress and chemotherapeutic treatment.PloS one · 2013Article
- Nucleostemin-like 1 is required for embryogenesis and leaf development in Arabidopsis.Plant molecular biology · 2012Article
- Decreased dyskerin levels as a mechanism of telomere shortening in X-linked dyskeratosis congenita.Journal of medical genetics · 2011Article
- Reactive oxygen species regulate nucleostemin oligomerization and protein degradation.The Journal of biological chemistry · 2011Article
- A strategy for full interrogation of prognostic gene expression patterns: exploring the biology of diffuse large B cell lymphoma.PloS one · 2011Article
- An off-target nucleostemin RNAi inhibits growth in human glioblastoma-derived cancer stem cells.PloS one · 2011Article
- A proteomic screen for nucleolar SUMO targets shows SUMOylation modulates the function of Nop5/Nop58.Molecular cell · 2010Article
- The dynamics of the alternatively spliced NOL7 gene products and role in nucleolar architecture.Nucleus (Austin, Tex.)Article
- Expression of nucleostemin in proliferating and differentiating cells of the human retina during prenatal development.Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sectionsArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Nucleostemin (NS) is a nucleolar protein involved in the regulation of cell proliferation. Both overexpression and knockdown of NS increase the activity of the tumor suppressor protein p53, resulting in cell cycle arrest. In addition, NS regulates processing of pre-rRNA and consequently the level of total protein synthesis. Here, we describe a previously uncharacterized function of NS in the maintenance of the tripartite nucleolar structure as well as the integrity of small nucleolar ribonucleoproteins (snoRNPs). NS is also necessary to maintain the telomerase complex which shares common protein subunits with the H/ACA box snoRNPs. First, immunofluorescence microscopy and electron microscopy demonstrated that knockdown of NS disorganized the nucleolar architecture, in particular, the dense fibrillar component where snoRNPs are localized. Second, gel filtration chromatography and immunoprecipitation indicated that NS depletion leads to dissociation of the components of snoRNPs and the telomerase complex. Third, NS depletion reduced both telomerase activity and the cellular level of pseudouridine, an H/ACA snoRNP-mediated modification of rRNA and other RNAs that are important for their folding and stability. These morphological, biochemical and functional studies demonstrate that NS plays an important role to maintain nucleolar structure and function on a more fundamental level than previously thought.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.