ArticleMolecular cell2009
Trypanosomes have six mitochondrial DNA helicases with one controlling kinetoplast maxicircle replication.
Article in Molecular cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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38 citing papers in PubMed, 71 citations in OpenAlex.
- A genome-wide genetic screen identifies a novel kDNA replication protein in trypanosomes.Nucleic acids research · 2026Article
- A trypanosome trifecta: an independently tunable triple inducible system for genetic studies inmSphere · 2026Article
- Identification of Cdc45-interacting partners uncovers two Leishmania donovani proteins involved in DNA replication.BMC microbiology · 2025Article
- From mitochondrial DNA arrangement to repair: a kinetoplast-associated protein with different roles in two trypanosomatid species.Parasites & vectors · 2025Article
- An independently tunable dual control system for RNAi complementation in Trypanosoma brucei.PloS one · 2025Article
- Pam16 and Pam18 were repurposed during Trypanosoma brucei evolution to regulate the replication of mitochondrial DNA.PLoS biology · 2024Article
- Mitochondrial genome maintenance-the kinetoplast story.FEMS microbiology reviews · 2023Review
- Pif1 Helicases and the Evidence for a Prokaryotic Origin of Helitrons.Molecular biology and evolution · 2022Article
- Article
- Conditional knockout of RAD51-related genes in Leishmania major reveals a critical role for homologous recombination during genome replication.PLoS genetics · 2020Article
- A DNA polymerization-independent role for mitochondrial DNA polymerase I-like protein C in African trypanosomes.Journal of cell science · 2020Article
- Article
- Cell cycle localization dynamics of mitochondrial DNA polymerase IC in African trypanosomes.Molecular biology of the cell · 2018Article
- Trypanosoma brucei UMSBP2 is a single-stranded telomeric DNA binding protein essential for chromosome end protection.Nucleic acids research · 2018Article
- Proteomic Analysis of the Cell Cycle of Procylic FormMolecular & cellular proteomics : MCP · 2018Article
- A glutaredoxin in the mitochondrial intermembrane space has stage-specific functions in the thermo-tolerance and proliferation of African trypanosomes.Redox biology · 2018Article
- A leucine aminopeptidase is involved in kinetoplast DNA segregation in Trypanosoma brucei.PLoS pathogens · 2017Article
- Review
- Elevation of Pollen Mitochondrial DNA Copy Number by WHIRLY2: Altered Respiration and Pollen Tube Growth in Arabidopsis.Plant physiology · 2015Article
- Detecting Significant Changes in Protein Abundance.EuPA open proteomics · 2015Article
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8 authors at 2 institutions in 2 countries.
Funding
Abstract
Kinetoplast DNA (kDNA), the trypanosome mitochondrial DNA, contains thousands of minicircles and dozens of maxicircles interlocked in a giant network. Remarkably, Trypanosoma brucei's genome encodes 8 PIF1-like helicases, 6 of which are mitochondrial. We now show that TbPIF2 is essential for maxicircle replication. Maxicircle abundance is controlled by TbPIF2 level, as RNAi of this helicase caused maxicircle loss, and its overexpression caused a 3- to 6-fold increase in maxicircle abundance. This regulation of maxicircle level is mediated by the TbHslVU protease. Previous experiments demonstrated that RNAi knockdown of TbHslVU dramatically increased abundance of minicircles and maxicircles, presumably because a positive regulator of their synthesis escaped proteolysis and allowed synthesis to continue. Here, we found that TbPIF2 level increases following RNAi of the protease. Therefore, this helicase is a TbHslVU substrate and an example of a positive regulator, thus providing a molecular mechanism for controlling maxicircle replication.
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