ArticleVeterinary microbiology2010
Development and use of real-time PCR to detect and quantify Mycoplasma haemocanis and "Candidatus Mycoplasma haematoparvum" in dogs.
Article in Veterinary microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 94 citations in OpenAlex.
- A naturally occurring feline leukaemia virus subgroup A variant exhibits broad mammalian cellular tropism and utilizes canine thiamine transporter 1.The Journal of general virology · 2026Article
- Molecular detection of hemotropic mycoplasmas (hemoplasmas) in humans and dogs living on islands and the seashore mainland of Brazil: a One Health approach.Parasites & vectors · 2026Article
- High prevalence of vector-borne protozoa and bacteria in dogs from Chad determined using a targeted next-generation sequencing approach.Scientific reports · 2025Article
- Vector-borne pathogens in dogs from the Republic of Kosovo.Parasites & vectors · 2025Article
- Apolipoprotein A-1 does not appear to be a suitable acute-phase reaction marker in canine babesiosis and hemoplasmosis.Current research in parasitology & vector-borne diseases · 2025Article
- Diversity of Ticks in the Caribbean Region and Detection of Their Pathogens Using BioMark Technology.Transboundary and emerging diseases · 2025Article
- Molecular study and phylogenetic analysis of hemotropic Mycoplasma in Baghdad province, Iraq.Open veterinary journal · 2024Article
- In Situ Treatment of Refractory Perianal Fistulas in Dogs with Low-Dose Allogeneic Adipose-Derived Mesenchymal Stem Cells.Animals : an open access journal from MDPI · 2024Article
- The detection of zoonotic microorganisms inFrontiers in veterinary science · 2024Article
- Next-generation sequencing metabarcoding assays reveal diverse bacterial vector-borne pathogens of Mongolian dogs.Current research in parasitology & vector-borne diseases · 2024Article
- Parasite Detection in Visceral Leishmaniasis Samples by Dye-Based qPCR Using New Gene Targets ofTropical medicine and infectious disease · 2023Article
- Transmission of haemotropic mycoplasma in the absence of arthropod vectors within a closed population of dogs on ectoparasiticides.Scientific reports · 2023Article
- High prevalence and genetic diversity of hemoplasmas in bats and bat ectoparasites from China.One health (Amsterdam, Netherlands) · 2023Article
- Molecular detection of hemoplasmas in rescued black-eared opossums (Didelphis aurita Wied-Neuwied, 1826) from southeastern Brazil, with evidence of a novel genotype infecting marsupials.Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria · 2023Article
- Occurrence of Mycoplasma parvum in German Pigs of Different Age Groups Using a Novel Quantitative Real-Time PCR AssayPathogens (Basel, Switzerland) · 2022Article
- Occurrence of 'Candidatus Mycoplasma haemosuis' in fattening pigs, sows and piglets in Germany using a novel gap-based quantitative real-time PCR assay.BMC veterinary research · 2022Article
- Novel High-Throughput Multiplex qPCRs for the Detection of Canine Vector-Borne Pathogens in the Asia-Pacific.Microorganisms · 2021Article
- Widespread Infection with Hemotropic Mycoplasmas in Free-Ranging Dogs and Wild Foxes Across Six Bioclimatic Regions of Chile.Microorganisms · 2021Article
- Molecular Survey and Genetic Diversity of Hemoplasmas in Rodents from Chile.Microorganisms · 2020Article
- Associations between clinical canine leishmaniosis and multiple vector-borne co-infections: a case-control serological study.BMC veterinary research · 2019Article
Corrections and comments
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Authors and funding
13 authors at 5 institutions in 2 countries.
Funding
Abstract
Two canine haemoplasma species have been recognised to date; Mycoplasma haemocanis (Mhc), which has been associated with anaemia in splenectomised or immunocompromised dogs, and "Candidatus Mycoplasma haematoparvum" (CMhp), recently described in an anaemic splenectomised dog undergoing chemotherapy. The study aim was to develop quantitative real-time PCR assays (qPCRs) incorporating an endogenous internal control to detect Mhc and CMhp and to apply these assays to DNA samples extracted from canine blood collected in Northern Tanzania (n=100) and from dogs presented to a Trinidadian veterinary hospital (n=185). QPCRs specific for Mhc and CMhp were designed using 16S rRNA gene sequence data, and each was duplexed with an assay specific for canine glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The assays detected < or =10 copies of a sequence-specific haemoplasma plasmid per reaction and neither assay showed cross-reactivity with 10(6) copies of the sequence-specific plasmid from the non-target canine haemoplasma species. Nineteen of the 100 Tanzanian samples (19%) were positive for Mhc alone and one (1%) was dually infected. One Trinidadian sample was negative for canine GAPDH DNA and was excluded from the study. Of the 184 remaining Trinidadian samples, nine (4.9%) were positive for Mhc alone, five (2.7%) for CMhp alone, and two (1.1%) dually infected. This is the first report of canine haemoplasma qPCR assays that use an internal control to confirm the presence of amplifiable sample DNA, and their application to prevalence studies. Mhc was the most commonly detected canine haemoplasma species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.