Evidence map›Paper›PMID 19646480›Full record

ArticleJournal of virological methods2009

Real time PCR TaqMan assays for detection of polyomaviruses KIV and WUV in clinical samples.

Massimiliano Bergallo, Maria Elena Terlizzi, Sara Astegiano, Marco Ciotti, Muhammed Babakir-Mina, Carlo Federico Perno, Rossana Cavallo, Cristina Costa

Abstract readEvaluation Study
In one paragraph

Article in Journal of virological methods, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Trial
  2. Article
  3. Review
  4. Article
  5. The occurrence of polyomaviruses WUPyV and KIPyV among patients with severe respiratory infections.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2019
    Article
  6. Article
  7. Article
  8. Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Massimiliano BergalloDepartment of Public Health and Microbiology, Virology Unit, University of Turin, Via Santena 9, 10126 Turin, Italy.
Maria Elena Terlizzi
Sara Astegiano
Marco Ciotti
Muhammed Babakir-Mina
Carlo Federico Perno
Rossana Cavallo
Cristina Costa

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Recently, polyomaviruses KI and WU were identified in the airways of patients with acute respiratory symptoms. The epidemiology and pathogenesis of these two viruses are not fully understood, and the development of molecular assays, such as Real Time PCR, was useful for examining their biology and role in different clinical syndromes. The evaluation of different target regions for the amplification of polyomaviruses KI and WU, comparing published primer/probe sets and sets designed in the laboratory is described and was used for testing 175 clinical specimens (84 stools and 91 tonsils). The results showed that the laboratory designs were more sensitive for the detection of polyomaviruses KI and WU DNA in clinical samples. The choice of the primer/probe set, and primarily of the region for amplification, may be relevant for understanding the pathogenic role of viruses such as polyomaviruses KI and WU.

Indexed as

PolyomavirusPolyomavirus InfectionsTumor Virus InfectionsDNA PrimersDNA, ViralFecesHumansPalatine TonsilReverse Transcriptase Polymerase Chain ReactionSensitivity and SpecificitySpecies SpecificityTaq PolymeraseDNA PrimersDNA, ViralTaq Polymerase

Identifiers

PMID19646480
PMCPMC7119675

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.