ArticleAlcoholism, clinical and experimental research2009
An assay for evoked locomotor behavior in Drosophila reveals a role for integrins in ethanol sensitivity and rapid ethanol tolerance.
Article in Alcoholism, clinical and experimental research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 35 citations in OpenAlex.
- Long term exposure to dietary cannabinoids inhibits rapid functional tolerance to ethanol inmicroPublication biology · 2025Article
- Large analysis of genetic manipulations reveals an inverse correlation between initial alcohol resistance and rapid tolerance phenotypes.Genes, brain, and behavior · 2024Article
- Large genetic analysis of alcohol resistance and tolerance reveals an inverse correlation and suggests 'true' tolerance mutants.bioRxiv : the preprint server for biology · 2023Article
- Methods for Exploring the Circuit Basis of Ethanol-Induced Changes inCold Spring Harbor protocols · 2023Article
- Ethanol Behavioral Responses inCold Spring Harbor protocols · 2023Article
- An Ant-Mimicking Jumping Spider Achieves Higher Predation Probability with Lower Success Rate When Exposed to Ethanol.Insects · 2022Article
- Repeated ethanol intoxications of Drosophila melanogaster adults increases the resistance to ethanol of their progeny.Alcoholism, clinical and experimental research · 2021Article
- Flying Together:International journal of molecular sciences · 2020Review
- Dietary yeast influences ethanol sedation in Drosophila via serotonergic neuron function.Addiction biology · 2020Article
- High-Throughput Method for Measuring Alcohol Sedation Time of Individual Drosophila melanogaster.Journal of visualized experiments : JoVE · 2020Article
- The Psychiatric Risk Gene NT5C2 Regulates Adenosine Monophosphate-Activated Protein Kinase Signaling and Protein Translation in Human Neural Progenitor Cells.Biological psychiatry · 2019Article
- Alcohol sedation in adultCommunications biology · 2019Article
- Genetics and genomics of alcohol responses in Drosophila.Neuropharmacology · 2017Review
- The allostatic impact of chronic ethanol on gene expression: A genetic analysis of chronic intermittent ethanol treatment in the BXD cohort.Alcohol (Fayetteville, N.Y.) · 2017Article
- Review
- Drosophila and Caenorhabditis elegans as Discovery Platforms for Genes Involved in Human Alcohol Use Disorder.Alcoholism, clinical and experimental research · 2015Review
- Rsu1 regulates ethanol consumption in Drosophila and humans.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- An inexpensive, scalable behavioral assay for measuring ethanol sedation sensitivity and rapid tolerance in Drosophila.Journal of visualized experiments : JoVE · 2015Article
- Contrasting influences of Drosophila white/mini-white on ethanol sensitivity in two different behavioral assays.Alcoholism, clinical and experimental research · 2014Article
- The FlyBar: administering alcohol to flies.Journal of visualized experiments : JoVE · 2014Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundEthanol induces similar behavioral responses in mammals and the fruit fly, Drosophila melanogaster. By coupling assays for ethanol-related behavior to the genetic tools available in flies, a number of genes have been identified that influence physiological responses to ethanol. To enhance the utility of the Drosophila model for investigating genes involved in ethanol-related behavior, we explored the value of an assay that measures the sedative effects of ethanol on negative geotaxis, an evoked locomotor response.
methodsWe established eRING (ethanol Rapid Iterative Negative Geotaxis) as an assay for quantitating the sedative effects of ethanol on negative geotaxis (i.e., startle-induced climbing). We validated the assay by assessing acute sensitivity to ethanol and rapid ethanol tolerance in several different control strains and in flies with mutations known to disrupt these behaviors. We also used eRING in a candidate screen to identify mutants with altered ethanol-related behaviors.
resultsNegative geotaxis measured in eRING assays was dose-dependently impaired by ethanol exposure. Flies developed tolerance to the intoxicating effects of ethanol when tested during a second exposure. Ethanol sensitivity and rapid ethanol tolerance varied across 4 control strains, but internal ethanol concentrations were indistinguishable in the 4 strains during a first and second challenge with ethanol. Ethanol sensitivity and rapid ethanol tolerance, respectively, were altered in flies with mutations in amnesiac and hangover, genes known to influence these traits. Additionally, mutations in the beta integrin gene myospheroid and the alpha integrin gene scab increased the initial sensitivity to ethanol and enhanced the development of rapid ethanol tolerance without altering internal ethanol concentrations.
conclusionsThe eRING assay is suitable for investigating genetic mechanisms that influence ethanol sensitivity and rapid ethanol tolerance. Ethanol sensitivity and rapid ethanol tolerance depend on the function of alpha and beta integrins in flies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.