ArticleThe Journal of molecular diagnostics : JMD2009
Rapid real-time PCR assays for detection of Klebsiella pneumoniae with the rmpA or magA genes associated with the hypermucoviscosity phenotype: screening of nonhuman primates.
Article in The Journal of molecular diagnostics : JMD, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 54 citations in OpenAlex.
- Efficacy of Moraceae with chlorhexidine mouthwash on the microbial flora of critically ill intubated patients: a randomized controlled pilot study.Scientific reports · 2022Trial
- Etiological and Pathological Investigation of Bacterial Pneumonia in Forest Musk Deer (Veterinary medicine international · 2026Article
- Genetic features of blaBMC microbiology · 2025Article
- Essential oils as capsule disruptors: enhancing antibiotic efficacy against multidrug-resistantFrontiers in microbiology · 2024Article
- Virulence, antimicrobial resistance, and molecular characteristics of carbapenem-resistant Klebsiella pneumoniae in a hospital in Shijiazhuang City from China.International microbiology : the official journal of the Spanish Society for Microbiology · 2023Article
- Crippling of Klebsiella pneumoniae virulence by metformin, N-acetylcysteine and secnidazole.BMC microbiology · 2023Article
- Detection and Quantification of Klebsiella pneumoniae in Fecal Samples Using Digital Droplet PCR in Comparison with Real-Time PCR.Microbiology spectrum · 2023Article
- Recombinant Reporter Phage rTUN1::ACS sensors · 2023Article
- Reduction of gastrointestinal tract colonization by Klebsiella quasipneumoniae using antimicrobial protein KvarIa.Gut pathogens · 2022Article
- Contamination of Hospital Surfaces with Bacterial Pathogens under the Current COVID-19 Outbreak.International journal of environmental research and public health · 2021Article
- An inter-laboratory study to investigate the impact of the bioinformatics component on microbiome analysis using mock communities.Scientific reports · 2021Article
- The ZKIR Assay, a Real-Time PCR Method for the Detection ofApplied and environmental microbiology · 2020Article
- Home Assessment of Indoor Microbiome (HAIM) in Relation to Lower Respiratory Tract Infections among Under-Five Children in Ibadan, Nigeria: The Study Protocol.International journal of environmental research and public health · 2020Article
- Rapid bacterial detection and antibiotic susceptibility testing in whole blood using one-step, high throughput blood digital PCR.Lab on a chip · 2020Article
- Speciation of common Gram-negative pathogens using a highly multiplexed high resolution melt curve assay.Scientific reports · 2018Article
- Genetic diversity of Klebsiella pneumoniae isolates during an outbreak in a non-human primate research colony.Journal of medical primatology · 2016Article
- Interaction of non-human primate complement and antibodies with hypermucoviscous Klebsiella pneumoniae.Veterinary research · 2016Article
- Quantitative analysis of pathogens in the lower respiratory tract of patients with chronic obstructive pulmonary disease.BMC pulmonary medicine · 2015Article
- Assessing the accuracy of quantitative molecular microbial profiling.International journal of molecular sciences · 2014Article
- Multiplex PCR for identification of two capsular types in epidemic KPC-producing Klebsiella pneumoniae sequence type 258 strains.Antimicrobial agents and chemotherapy · 2014Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The relationship of mucoviscosity-associated (magA) and/or regulator of mucoid phenotype (rmpA) genes to the Klebsiella pneumoniae hypermucoviscosity (HMV) phenotype has been reported. We previously demonstrated that rmpA+ K. pneumoniae can cause serious disease in African green monkeys and isolated rmpA+ and magA+ HMV K. pneumoniae from other species of non-human primates. To rapidly screen African green monkeys/non-human primates for these infections, we developed three real-time PCR assays. The first was K. pneumoniae-specific, targeting the khe gene, while the others targeted rmpA and magA. Primer Express 2 was used with the three K. pneumoniae genes to generate sequence-specific TaqMan/TaqMan-Minor Groove Binder assays. Oral/rectal swabs and necropsy samples were collected; swabs were used for routine culture and DNA extraction. K. pneumoniae colonies were identified on the Vitek 2 with DNA tested using the K. pneumoniae-specific assays. Testing of 45 African green monkeys resulted in 19 khe+ samples from 14 animals with none positive for either rmpA or magA. Of these 19 khe+ samples, five were culture-positive, but none were HMV "string test"-positive. Subsequent testing of 307 non-human primates resulted in 64 HMV K. pneumoniae isolates of which 42 were rmpA+ and 15 were magA+. Non-human primate testing at the U.S. Army Medical Research Institute of Infectious Diseases demonstrated the ability to screen both live and necropsied animals for K. pneumoniae by culture and real-time PCR to determine HMV genotype.
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