ArticleRNA (New York, N.Y.)2009
Activation of decapping involves binding of the mRNA and facilitation of the post-binding steps by the Lsm1-7-Pat1 complex.
Article in RNA (New York, N.Y.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 41 citations in OpenAlex.
- Multi-omics revealed that DCP1A and SPDL1 determine embryogenesis defects in postovulatory ageing oocytes.Cell proliferation · 2025Article
- Yeast Lsm Pro-Apoptotic Mutants Show Defects in Autophagy.International journal of molecular sciences · 2023Article
- Expression profile and prognostic values of LSM family in skin cutaneous melanoma.BMC medical genomics · 2022Article
- A novel mRNA decay inhibitor abolishes pathophysiological cellular transition.Cell death discovery · 2022Article
- Regulatory RNAs: A Universal Language for Inter-Domain Communication.International journal of molecular sciences · 2020Review
- Additional Layer of Regulation via Convergent Gene Orientation in Yeasts.Molecular biology and evolution · 2020Article
- Transcriptome-Wide Comparison of Stress Granules and P-Bodies Reveals that Translation Plays a Major Role in RNA Partitioning.Molecular and cellular biology · 2019Article
- The Pat1-Lsm Complex Stabilizes ATG mRNA during Nitrogen Starvation-Induced Autophagy.Molecular cell · 2019Article
- The Lsm1-7/Pat1 complex binds to stress-activated mRNAs and modulates the response to hyperosmotic shock.PLoS genetics · 2018Article
- Molecular architecture of LSM14 interactions involved in the assembly of mRNA silencing complexes.The EMBO journal · 2018Article
- Beyond transcription factors: roles of mRNA decay in regulating gene expression in plants.F1000Research · 2018Review
- Structural basis of mRNA-cap recognition by Dcp1-Dcp2.Nature structural & molecular biology · 2016Article
- The Lsm1-7-Pat1 complex promotes viral RNA translation and replication by differential mechanisms.RNA (New York, N.Y.) · 2015Article
- Genetic analysis of resistance and sensitivity to 2-deoxyglucose in Saccharomyces cerevisiae.Genetics · 2014Article
- Pat1 contributes to the RNA binding activity of the Lsm1-7-Pat1 complex.RNA (New York, N.Y.) · 2014Article
- The C-terminal domain from S. cerevisiae Pat1 displays two conserved regions involved in decapping factor recruitment.PloS one · 2014Article
- The role of disordered protein regions in the assembly of decapping complexes and RNP granules.Genes & development · 2013Review
- P-bodies and stress granules: possible roles in the control of translation and mRNA degradation.Cold Spring Harbor perspectives in biology · 2012Review
- Both Sm-domain and C-terminal extension of Lsm1 are important for the RNA-binding activity of the Lsm1-7-Pat1 complex.RNA (New York, N.Y.) · 2012Article
- Dcp1 links coactivators of mRNA decapping to Dcp2 by proline recognition.RNA (New York, N.Y.) · 2011Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Decapping is a critical step in the conserved 5'-to-3' mRNA decay pathway of eukaryotes. The hetero-octameric Lsm1-7-Pat1 complex is required for normal rates of decapping in this pathway. This complex also protects the mRNA 3'-ends from trimming in vivo. To elucidate the mechanism of decapping, we analyzed multiple lsm1 mutants, lsm1-6, lsm1-8, lsm1-9, and lsm1-14, all of which are defective in decapping and 3'-end protection but unaffected in Lsm1-7-Pat1 complex integrity. The RNA binding ability of the mutant complex was found to be almost completely lost in the lsm1-8 mutant but only partially impaired in the other mutants. Importantly, overproduction of the Lsm1-9p- or Lsm1-14p-containing (but not Lsm1-8p-containing) mutant complexes in wild-type cells led to a dominant inhibition of mRNA decay. Further, the mRNA 3'-end protection defect of lsm1-9 and lsm1-14 cells, but not the lsm1-8 cells, could be partly suppressed by overproduction of the corresponding mutant complexes in those cells. These results suggest the following: (1) Decapping requires both binding of the Lsm1-7-Pat1 complex to the mRNA and facilitation of the post-binding events, while binding per se is sufficient for 3'-end protection. (2) A major block exists at the post-binding steps in the lsm1-9 and lsm1-14 mutants and at the binding step in the lsm1-8 mutant. Consistent with these ideas, the lsm1-9, 14 allele generated by combining the mutations of lsm1-9 and lsm1-14 alleles had almost fully lost the RNA binding activity of the complex and behaved like the lsm1-8 mutant.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.