ArticleJournal of magnetic resonance (San Diego, Calif. : 1997)2009
EPIC- and CHANCE-HSQC: two 15N-photo-CIDNP-enhanced pulse sequences for the sensitive detection of solvent-exposed tryptophan.
Article in Journal of magnetic resonance (San Diego, Calif. : 1997), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed.
- Nuclear-Spin Hyperpolarization of Biomolecules by Optically Enhanced NMR at Ultra-High Field.Journal of the Chinese Chemical Society. Zhongguo hua xue hui (Taipei, Taiwan) · 2026Article
- Dynamic nuclear polarization and chemically induced hyperpolarization: Progress, mechanisms, and opportunities.Magnetic resonance letters · 2025Review
- Detection of sub-nmol amounts of the antiviral drug favipiravir inScientific reports · 2024Article
- Spin Hyperpolarization in Modern Magnetic Resonance.Chemical reviews · 2023Review
- Improved sensitivity of laser-enhancedJournal of magnetic resonance (San Diego, Calif. : 1997) · 2019Article
- Pushing nuclear magnetic resonance sensitivity limits with microfluidics and photo-chemically induced dynamic nuclear polarization.Nature communications · 2018Article
- Effect of heavy atoms on photochemically induced dynamic nuclear polarization in liquids.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2018Article
- Fluorescein: A Photo-CIDNP Sensitizer Enabling Hypersensitive NMR Data Collection in Liquids at Low Micromolar Concentration.The journal of physical chemistry. B · 2016Article
- Sensitivity enhancement in solution NMR: emerging ideas and new frontiers.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2014Review
- A novel tri-enzyme system in combination with laser-driven NMR enables efficient nuclear polarization of biomolecules in solution.The journal of physical chemistry. B · 2013Article
- 1H-Detected 13C photo-CIDNP as a sensitivity enhancement tool in solution NMR.Journal of the American Chemical Society · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Photochemically induced dynamic nuclear polarization (photo-CIDNP) of nuclei other than (1)H offers a tremendous potential for sensitivity enhancement in liquid state NMR under mild, physiologically relevant conditions. Photo-CIDNP enhancements of (15)N magnetization are much larger than those typically observed for (1)H. However, the low gyromagnetic ratio of (15)N prevents a full fruition of the potential signal-to-noise gains attainable via (15)N photo-CIDNP. Here, we propose two novel pulse sequences, EPIC- and CHANCE-HSQC, tailored to overcome the above limitation. EPIC-HSQC exploits the strong (1)H polarization and its subsequent transfer to non-equilibrium N(z) magnetization prior to (15)N photo-CIDNP laser irradiation. CHANCE-HSQC synergistically combines (1)H and (15)N photo-CIDNP. The above pulse sequences, tested on tryptophan (Trp) and the Trp-containing protein apoHmpH, were found to display up to 2-fold higher sensitivity than the reference NPE-SE-HSQC pulse train (based on simple (15)N photo-CIDNP followed by N-H polarization transfer), and up to a ca. 3-fold increase in sensitivity over the corresponding dark pulse schemes (lacking laser irradiation). The observed effects are consistent with the predictions from a theoretical model of photo-CIDNP and prove the potential of (15)N and (1)H photo-CIDNP in liquid state heteronuclear correlation NMR.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.