ArticleBMC plant biology2009
Mass spectrometry analysis of the variants of histone H3 and H4 of soybean and their post-translational modifications.
Article in BMC plant biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 47 citations in OpenAlex.
- New Histone H4 variant and H2B variant Exhibits distinct genomic distributions, chromatin affinities, and dynamics throughout life and cell cycle of Trypanosoma cruzi.PLoS pathogens · 2026Article
- The plant unique ESCRT component FREE1 regulates autophagosome closure.Nature communications · 2023Article
- Ufmylation reconciles salt stress-induced unfolded protein responses via ER-phagy inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Trypanosoma brucei histones are heavily modified with combinatorial post-translational modifications and mark Pol II transcription start regions with hyperacetylated H2A.Nucleic acids research · 2022Article
- Methylation hallmarks on the histone tail as a linker of osmotic stress and gene transcription.Frontiers in plant science · 2022Review
- Not all Is SET for Methylation: Evolution of Eukaryotic Protein Methyltransferases.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Cataloging Posttranslational Modifications in Plant Histones.Advances in experimental medicine and biology · 2021Article
- Genome-wide comparative analysis of DNA methylation between soybean cytoplasmic male-sterile line NJCMS5A and its maintainer NJCMS5B.BMC genomics · 2017Article
- Analysis of Histones H3 and H4 Reveals Novel and Conserved Post-Translational Modifications in Sugarcane.PloS one · 2015Article
- Proteomics and Metabolomics: Two Emerging Areas for Legume Improvement.Frontiers in plant science · 2015Review
- Comprehending dynamic protein methylation with mass spectrometry.Current opinion in chemical biology · 2013Review
- Chemical and biochemical approaches in the study of histone methylation and demethylation.Medicinal research reviews · 2012Review
- The Golgi-localized Arabidopsis endomembrane protein12 contains both endoplasmic reticulum export and Golgi retention signals at its C terminus.The Plant cell · 2012Article
- GmPHD5 acts as an important regulator for crosstalk between histone H3K4 di-methylation and H3K14 acetylation in response to salinity stress in soybean.BMC plant biology · 2011Article
- Regulation by polycomb and trithorax group proteins in Arabidopsis.The arabidopsis book · 2010Article
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7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHistone modifications and histone variants are of importance in many biological processes. To understand the biological functions of the global dynamics of histone modifications and histone variants in higher plants, we elucidated the variants and post-translational modifications of histones in soybean, a legume plant with a much bigger genome than that of Arabidopsis thaliana.
resultsIn soybean leaves, mono-, di- and tri-methylation at Lysine 4, Lysine 27 and Lysine 36, and acetylation at Lysine 14, 18 and 23 were detected in HISTONE H3. Lysine 27 was prone to being mono-methylated, while tri-methylation was predominant at Lysine 36. We also observed that Lysine 27 methylation and Lysine 36 methylation usually excluded each other in HISTONE H3. Although methylation at HISTONE H3 Lysine 79 was not reported in A. thaliana, mono- and di-methylated HISTONE H3 Lysine 79 were detected in soybean. Besides, acetylation at Lysine 8 and 12 of HISTONE H4 in soybean were identified. Using a combination of mass spectrometry and nano-liquid chromatography, two variants of HISTONE H3 were detected and their modifications were determined. They were different at positions of A31F41S87S90 (HISTONE variant H3.1) and T31Y41H87L90 (HISTONE variant H3.2), respectively. The methylation patterns in these two HISTONE H3 variants also exhibited differences. Lysine 4 and Lysine 36 methylation were only detected in HISTONE H3.2, suggesting that HISTONE variant H3.2 might be associated with actively transcribing genes. In addition, two variants of histone H4 (H4.1 and H4.2) were also detected, which were missing in other organisms. In the histone variant H4.1 and H4.2, the amino acid 60 was isoleucine and valine, respectively.
conclusionThis work revealed several distinct variants of soybean histone and their modifications that were different from A. thaliana, thus providing important biological information toward further understanding of the histone modifications and their functional significance in higher plants.
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