ArticleEuropean biophysics journal : EBJ2010
Cholesterol modulation of nicotinic acetylcholine receptor surface mobility.
Article in European biophysics journal : EBJ, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 45 citations in OpenAlex.
- Five key challenges for the plasma membrane insertion of nicotinic acetylcholine receptors in cellular model systems.Molecular and cellular biochemistry · 2026Review
- Modulation of a rapid neurotransmitter receptor-ion channel by membrane lipids.Frontiers in cell and developmental biology · 2023Review
- Fluorescence Studies of Nicotinic Acetylcholine Receptor and Its Associated Lipid Milieu: The Influence of Erwin London's Methodological Approaches.The Journal of membrane biology · 2022Review
- Interactions between the Nicotinic and Endocannabinoid Receptors at the Plasma Membrane.Membranes · 2022Review
- Chronic cholesterol depletion increases F-actin levels and induces cytoskeletal reorganization via a dual mechanism.Journal of lipid research · 2022Article
- Fluorescence microscopy imaging of a neurotransmitter receptor and its cell membrane lipid milieu.Frontiers in molecular biosciences · 2022Review
- Nanoscale Sub-Compartmentalization of the Dendritic Spine Compartment.Biomolecules · 2021Review
- Dysregulation of Neuronal Nicotinic Acetylcholine Receptor-Cholesterol Crosstalk in Autism Spectrum Disorder.Frontiers in molecular neuroscience · 2021Article
- Depletion of the cellular cholesterol content reduces the dynamics of desmosomal cadherins and interferes with desmosomal strength.Histochemistry and cell biology · 2019Article
- Motional dynamics of single Patched1 molecules in cilia are controlled by Hedgehog and cholesterol.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Alzheimer's Disease as a Membrane Disorder: Spatial Cross-Talk Among Beta-Amyloid Peptides, Nicotinic Acetylcholine Receptors and Lipid Rafts.Frontiers in cellular neuroscience · 2019Review
- Cholesterol modulates acetylcholine receptor diffusion by tuning confinement sojourns and nanocluster stability.Scientific reports · 2018Article
- Biased μ-opioid receptor agonists diversely regulate lateral mobility and functional coupling of the receptor to its cognate G proteins.Naunyn-Schmiedeberg's archives of pharmacology · 2016Article
- Lateral diffusion, function, and expression of the slow channel congenital myasthenia syndrome αC418W nicotinic receptor mutation with changes in lipid raft components.The Journal of biological chemistry · 2015Article
- Cell-surface translational dynamics of nicotinic acetylcholine receptors.Frontiers in synaptic neuroscience · 2014Review
- Membrane domain formation-a key factor for targeted intracellular drug delivery.Frontiers in physiology · 2014Review
- Transient cholesterol effects on nicotinic acetylcholine receptor cell-surface mobility.PloS one · 2014Article
- A distinct mechanism for activating uncoupled nicotinic acetylcholine receptors.Nature chemical biology · 2013Article
- Contribution of α7 nicotinic receptor to airway epithelium dysfunction under nicotine exposure.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Molecular mechanisms of acetylcholine receptor-lipid interactions: from model membranes to human biology.Biophysical reviews · 2013Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nicotinic acetylcholine receptor (AChR) function and distribution are quite sensitive to cholesterol (Chol) levels in the plasma membrane (reviewed by Barrantes in J Neurochem 103 (suppl 1):72-80, 2007). Here we combined confocal fluorescence recovery after photobleaching (FRAP) and confocal fluorescence correlation spectroscopy (FCS) to examine the mobility of the AChR and its dependence on Chol content at the cell surface of a mammalian cell line. Plasma membrane AChR exhibited limited mobility and only ~55% of the fluorescence was recovered within 10 min after photobleaching. Depletion of membrane Chol by methyl-beta-cyclodextrin strongly affected the mobility of the AChR at the plasma membrane; the fraction of mobile AChR fell from 55 to 20% in Chol-depleted cells, whereas Chol enrichment by methyl-beta-cyclodextrin-Chol treatment did not reduce receptor mobility at the cell surface. Actin depolymerization caused by latrunculin A partially restored receptor mobility in Chol-depleted cells. In agreement with the FRAP data, scanning FCS experiments showed that the diffusion coefficient of the AChR was about 30% lower upon Chol depletion. Taken together, these results suggest that membrane Chol modulates AChR mobility at the plasma membrane through a Chol-dependent mechanism sensitive to cortical actin.
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