ArticleBiology of reproduction2009
Production of germline transgenic prairie voles (Microtus ochrogaster) using lentiviral vectors.
Article in Biology of reproduction, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Characterizing Microglia Morphology in the Frontal Cortex of Pair-Bonded and Unpaired Prairie Voles (International journal of molecular sciences · 2025Article
- Lentiviral CRISPRa/i in the adult prairie vole brain: modulating neuronal gene expression without DNA cleavage.Frontiers in genome editing · 2025Article
- The effects of different types of social interactions on the electrophysiology of neurons in the nucleus accumbens in rodents.Neuroscience and biobehavioral reviews · 2024Review
- Emergent intra-pair sex differences and organized behavior in pair bonded prairie voles (Microtus ochrogaster).Genes, brain, and behavior · 2022Article
- How prior pair-bonding experience affects future bonding behavior in monogamous prairie voles.Hormones and behavior · 2020Article
- Assisted reproductive technologies inReproductive medicine and biology · 2019Review
- Neural Circuits Underlying Rodent Sociality: A Comparative Approach.Current topics in behavioral neurosciences · 2019Article
- Neuropeptidergic regulation of pair-bonding and stress buffering: Lessons from voles.Hormones and behavior · 2015Review
- Oxytocin and vasopressin in children and adolescents with autism spectrum disorders: sex differences and associations with symptoms.Autism research : official journal of the International Society for Autism Research · 2013Article
- Variation in vasopressin receptor (Avpr1a) expression creates diversity in behaviors related to monogamy in prairie voles.Hormones and behavior · 2013Article
- Amniotic fluid derived stem cells give rise to neuron-like cells without a further differentiation potential into retina-like cells.American journal of stem cells · 2013Article
- Identification of variables contributing to superovulation efficiency for production of transgenic prairie voles (Microtus ochrogaster).Reproductive biology and endocrinology : RB&E · 2012Article
- Article
- The prairie vole: an emerging model organism for understanding the social brain.Trends in neurosciences · 2010Article
- Development of genomic resources for the prairie vole (Microtus ochrogaster): construction of a BAC library and vole-mouse comparative cytogenetic map.BMC genomics · 2010Article
- We're the Same... but Different: Addressing Academic Divides in the Study of Brain and Behavior.Frontiers in behavioral neuroscience · 2010Article
- Toward an integrative understanding of social behavior: new models and new opportunities.Frontiers in behavioral neuroscience · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The study of alternative model organisms has yielded tremendous insights into the regulation of behavioral and physiological traits not displayed by more widely used animal models, such as laboratory rats and mice. In particular, comparative approaches often exploit species ideally suited for investigating specific phenomenon. For instance, comparative studies of socially monogamous prairie voles and polygamous meadow voles have been instrumental toward gaining an understanding of the genetic and neurobiological basis of social bonding. However, laboratory studies of less commonly used organisms, such as prairie voles, have been limited by a lack of genetic tools, including the ability to manipulate the genome. Here, we show that lentiviral vector-mediated transgenesis is a rapid and efficient approach for creating germline transgenics in alternative laboratory rodents. Injection of a green fluorescent protein (GFP)-expressing lentiviral vector into the perivitelline space of 23 single-cell embryos yielded three live offspring (13 %), one of which (33%) contained germline integration of a GFP transgene driven by the human ubiquitin-C promoter. In comparison, transfer of 23 uninjected embryos yielded six live offspring (26%). Green fluorescent protein is present in all tissues examined and is expressed widely in the brain. The GFP transgene is heritable and stably expressed until at least the F(2) generation. This technology has the potential to allow investigation of specific gene candidates in prairie voles and provides a general protocol to pursue germline transgenic manipulation in many different rodent species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.