ArticleClinical and vaccine immunology : CVI2009
Development and evaluation of an enzyme-linked immunosorbent assay based on recombinant VP2 capsids for the detection of antibodies to Aleutian mink disease virus.
Article in Clinical and vaccine immunology : CVI, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 44 citations in OpenAlex.
- A novel high-sensitivity TaqMan qPCR assay reveals amdoparvovirus DNA in zoo-housed small mammals in southern China.Veterinary research communications · 2026Article
- Strong selection signatures for Aleutian disease tolerance acting on novel candidate genes linked to immune and cellular responses in American mink (Neogale vison).Scientific reports · 2024Article
- Aleutian disease: Risk factors and ImmunAD strategy for genetic improvement of tolerance in American mink (Neogale vison).PloS one · 2024Article
- A developed TaqMan probe-based qPCR was used to quantify the distribution of AMDV in various tissues of infected mink and its prevalence in northern China.Frontiers in veterinary science · 2024Article
- Identifying selection signatures for immune response and resilience to Aleutian disease in mink using genotype data.Frontiers in genetics · 2024Article
- Review
- Asymptomatic viral infection is associated with lower host reproductive output in wild mink populations.Scientific reports · 2023Article
- Genetic and phenotypic correlations between Aleutian disease tests with body weight, growth, and feed efficiency traits in mink.Journal of animal science · 2022Article
- Applying Machine Learning Algorithms for the Classification of Mink Infected with Aleutian Disease Using Different Data Sources.Animals : an open access journal from MDPI · 2022Article
- Seroprevalence and Molecular Epidemiology of Aleutian Disease in Various Countries during 1972-2021: A Review and Meta-Analysis.Animals : an open access journal from MDPI · 2021Review
- AMDV Vaccine: Challenges and Perspectives.Viruses · 2021Review
- Diversity and transmission of Aleutian mink disease virus in feral and farmed American mink and native mustelids.Virus evolution · 2021Article
- Development of an antigen-capture enzyme-linked immunosorbent assay for diagnosis of Aleutian mink disease virus.Archives of virology · 2021Article
- Questionnaire survey of detrimental fur animal epidemic necrotic pyoderma in Finland.Acta veterinaria Scandinavica · 2017Article
- Genetic characterization of the complete genome of an Aleutian mink disease virus isolated in north China.Virus genes · 2016Article
- Development of an Enzyme-Linked Immunosorbent Assay Based on Fusion VP2332-452 Antigen for Detecting Antibodies against Aleutian Mink Disease Virus.Journal of clinical microbiology · 2016Article
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- Validation of an automated ELISA system for detection of antibodies to Aleutian mink disease virus using blood samples collected in filter paper strips.Virology journal · 2014Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aleutian disease (AD), a common infectious disease in farmed minks worldwide, is caused by Aleutian mink disease virus (AMDV). Serodiagnosis of AD in minks has been based on detection of AMDV antibodies by counterimmunoelectrophoresis (CIE) since the 1980s. The aim of this study was to develop and evaluate an enzyme-linked immunosorbent assay (ELISA) based on recombinant virus-like particles (VLPs) for identifying AMDV antibodies from mink sera. AMDV capsid protein (VP2) of a Finnish wild-type strain was expressed by the baculovirus system in Spodoptera frugiperda 9 insect cells and was shown to self-assemble to VLPs (with an ultrastructure similar to that of the actual virion). A direct immunoglobulin G ELISA was established using purified recombinant AMDV VP2 VLPs as an antigen. Sera from farmed minks were collected to evaluate the AMDV VP2 ELISA (n = 316) and CIE (n = 209) based on AMDV VP2 recombinant antigen in parallel with CIE performed using a commercially available traditional antigen. CIE performed with the recombinant antigen had a sensitivity and specificity of 100% and ELISA a sensitivity of 99% and a specificity of 97%, with reference to CIE performed with the commercial antigen. The results show that the recombinant AMDV VP2 VLPs are antigenic and that AMDV VP2 ELISA is sensitive and specific and encourage further development of the method for high-throughput diagnostics, involving hundreds of thousands of samples in Finland annually.
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