ArticleClinical and vaccine immunology : CVI2009
Identification of novel Mycobacterium bovis antigens by dissection of crude protein fractions.
Article in Clinical and vaccine immunology : CVI, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed, 15 citations in OpenAlex.
- Animal models for COVID-19 and tuberculosis.Frontiers in immunology · 2023Review
- Comparison of Cattle Serum Antibody Responses to Five Different Mycobacterial Antigens in an ELISA System.Archives of Razi Institute · 2021Article
- Detection and characterization of purified antigenic proteins from culture filtrate ofIranian journal of microbiology · 2020Article
- Evolutionary history and global spread of the Mycobacterium tuberculosis Beijing lineage.Nature genetics · 2015Article
- Evaluation of cocktails with recombinant proteins of Mycobacterium bovis for a specific diagnosis of bovine tuberculosis.BioMed research international · 2014Article
- Screening of recombinant proteins as antigens in indirect ELISA for diagnosis of bovine tuberculosis.SpringerPlus · 2012Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Culture filtrate and cell extracts from Mycobacterium bovis cultures contain molecules which could promote protective immunity to tuberculosis in animals. Different protein fractions of M. bovis cultures were obtained by elution electrophoresis and were tested in experimentally infected cattle. The fractions that elicited gamma interferon (IFN-gamma) responses were resolved by two-dimensional gel electrophoresis, and individual proteins were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry. The open reading frames were cloned, expressed as their recombinant forms, and retested with naturally and experimentally infected animals. Eleven protein fractions were highly reactive, from which the Rv1636, HspX, Rv0138, Rv2524, EsxI, and Rv3740 recombinant proteins were obtained. EsxI and HspX were the antigens most recognized by the IFN-gamma release assay. In summary, a proteomic approach allowed the identification of novel antigens useful for the diagnosis of bovine tuberculosis.
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Registered trials
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