ArticlePlant physiology2009
TILLING in Lotus japonicus identified large allelic series for symbiosis genes and revealed a bias in functionally defective ethyl methanesulfonate alleles toward glycine replacements.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 94 citations in OpenAlex.
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- Comprehensive Quantification of (Poly)phenols inJournal of agricultural and food chemistry · 2025Article
- Untargeted metabolomics reveals novel metabolites in Lotus japonicus roots during arbuscular mycorrhiza symbiosis.The New phytologist · 2025Article
- A Suite of Pea (International journal of molecular sciences · 2025Article
- Ethylene promotes SMAX1 accumulation to inhibit arbuscular mycorrhiza symbiosis.Nature communications · 2025Article
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- Novel gene loci associated with susceptibility or cryptic quantitative resistance to Pyrenopeziza brassicae in Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- NIN-at the heart of NItrogen-fixing Nodule symbiosis.Frontiers in plant science · 2023Review
- Is it the end of TILLING era in plant science?Frontiers in plant science · 2023Review
- Lotus japonicus Nuclear Factor YA1, a nodule emergence stage-specific regulator of auxin signalling.The New phytologist · 2021Article
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- An Efficient Protocol for Model Legume Root Protoplast Isolation and Transformation.Frontiers in plant science · 2018Article
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- A quantitative hypermorphiceLife · 2017Article
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- Lotus Base: An integrated information portal for the model legume Lotus japonicus.Scientific reports · 2016Article
- Hemoglobin LjGlb1-1 is involved in nodulation and regulates the level of nitric oxide in the Lotus japonicus-Mesorhizobium loti symbiosis.Journal of experimental botany · 2016Article
- Efficient Inactivation of Symbiotic Nitrogen Fixation Related Genes in Lotus japonicus Using CRISPR-Cas9.Frontiers in plant science · 2016Article
- Identification of altered metabolic pathways of γ-irradiated rice mutant via network-based transcriptome analysis.Genetica · 2015Article
- SCARN a Novel Class of SCAR Protein That Is Required for Root-Hair Infection during Legume Nodulation.PLoS genetics · 2015Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
We have established tools for forward and reverse genetic analysis of the legume Lotus (Lotus japonicus). A structured population of M2 progeny of 4,904 ethyl methanesulfonate-mutagenized M1 embryos is available for single nucleotide polymorphism mutation detection, using a TILLING (for Targeting Induced Local Lesions IN Genomes) protocol. Scanning subsets of this population, we identified a mutation load of one per 502 kb of amplified fragment. Moreover, we observed a 1:10 ratio between homozygous and heterozygous mutations in the M2 progeny. This reveals a clear difference in germline genetics between Lotus and Arabidopsis (Arabidopsis thaliana). In addition, we assembled M2 siblings with obvious phenotypes in overall development, starch accumulation, or nitrogen-fixing root nodule symbiosis in three thematic subpopulations. By screening the nodulation-defective population of M2 individuals for mutations in a set of 12 genes known to be essential for nodule development, we identified large allelic series for each gene, generating a unique data set that combines genotypic and phenotypic information facilitating structure-function studies. This analysis revealed a significant bias for replacements of glycine (Gly) residues in functionally defective alleles, which may be explained by the exceptional structural features of Gly. Gly allows the peptide chain to adopt conformations that are no longer possible after amino acid replacement. This previously unrecognized vulnerability of proteins at Gly residues could be used for the improvement of algorithms that are designed to predict the deleterious nature of single nucleotide polymorphism mutations. Our results demonstrate the power, as well as the limitations, of ethyl methanesulfonate mutagenesis for forward and reverse genetic studies. (Original mutant phenotypes can be accessed at http://data.jic.bbsrc.ac.uk/cgi-bin/lotusjaponicus Access to the Lotus TILLING facility can be obtained through http://www.lotusjaponicus.org or http://revgenuk.jic.ac.uk).
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