ArticleMolecular biology of the cell2009
Transportin regulates major mitotic assembly events: from spindle to nuclear pore assembly.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 60 citations in OpenAlex.
- Structure, function and assembly of nuclear pore complexes.Nature reviews. Molecular cell biology · 2026Review
- Cytoplasmic nucleoporin assemblage: the cellular artwork in physiology and disease.Nucleus (Austin, Tex.) · 2024Review
- Reconstitution of nuclear envelope subdomain formation on mitotic chromosomes in semi-intact cells.Cell structure and function · 2024Article
- Nucleoporin Elys attaches peripheral chromatin to the nuclear pores in interphase nuclei.Communications biology · 2024Article
- Nuclear transport proteins: structure, function, and disease relevance.Signal transduction and targeted therapy · 2023Review
- Attenuated Viral Replication of AvianMicrobiology spectrum · 2022Article
- Review
- Visualizing Nuclear Pore Complexes in Xenopus Egg Extracts.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The Role of Nucleoporin Elys in Nuclear Pore Complex Assembly and Regulation of Genome Architecture.International journal of molecular sciences · 2020Review
- Exportins can inhibit major mitotic assembly eventsNucleus (Austin, Tex.) · 2020Article
- Nup358 and Transportin 1 Cooperate in Adenoviral Genome Import.Journal of virology · 2020Article
- Structural and biochemical analyses of the nuclear pore complex component ELYS identify residues responsible for nucleosome binding.Communications biology · 2019Article
- Molecular dissection of an inhibitor targeting the HIV integrase dependent preintegration complex nuclear import.Cellular microbiology · 2019Article
- Inhibition of the acetyltransferase NAT10 normalizes progeric and aging cells by rebalancing the Transportin-1 nuclear import pathway.Science signaling · 2018Article
- Distinctive Nuclear Localization Signals in the OomyceteFrontiers in microbiology · 2017Article
- Identification of potential cargo proteins of transportin protein AtTRN1 in Arabidopsis thaliana.Plant cell reports · 2016Article
- Nucleosome functions in spindle assembly and nuclear envelope formation.BioEssays : news and reviews in molecular, cellular and developmental biology · 2015Review
- Nuclear transport factors: global regulation of mitosis.Current opinion in cell biology · 2015Review
- The RanGTP Pathway: From Nucleo-Cytoplasmic Transport to Spindle Assembly and Beyond.Frontiers in cell and developmental biology · 2015Review
- Purification of nuclear localization signal-containing proteins and its application to investigation of the mechanisms of the cell division cycle.Small GTPases · 2015Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Mitosis in higher eukaryotes is marked by the sequential assembly of two massive structures: the mitotic spindle and the nucleus. Nuclear assembly itself requires the precise formation of both nuclear membranes and nuclear pore complexes. Previously, importin alpha/beta and RanGTP were shown to act as dueling regulators to ensure that these assembly processes occur only in the vicinity of the mitotic chromosomes. We now find that the distantly related karyopherin, transportin, negatively regulates nuclear envelope fusion and nuclear pore assembly in Xenopus egg extracts. We show that transportin-and importin beta-initiate their regulation as early as the first known step of nuclear pore assembly: recruitment of the critical pore-targeting nucleoporin ELYS/MEL-28 to chromatin. Indeed, each karyopherin can interact directly with ELYS. We further define the nucleoporin subunit targets for transportin and importin beta and find them to be largely the same: ELYS, the Nup107/160 complex, Nup53, and the FG nucleoporins. Equally importantly, we find that transportin negatively regulates mitotic spindle assembly. These negative regulatory events are counteracted by RanGTP. We conclude that the interplay of the two negative regulators, transportin and importin beta, along with the positive regulator RanGTP, allows precise choreography of multiple cell cycle assembly events.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.