ArticleMolecular biology of the cell2009
Dissection of CENP-C-directed centromere and kinetochore assembly.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 94 papers.
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Who cites it
94 citing papers in PubMed, 137 citations in OpenAlex.
- Structure and function of the centromere and inner kinetochore.Nature reviews. Molecular cell biology · 2026Review
- The CENP-A chaperone complex spatially organizes centromeres.Science advances · 2026Article
- Phosphorylation of Xenopus M18BP1 governs centromeric localization and CENP-A nucleosome assembly.EMBO reports · 2026Article
- Maternal CENP-C restores centromere symmetry in mammalian zygotes to ensure proper chromosome segregation.Developmental cell · 2026Article
- Article
- The CENP-A chaperone complex spatially organizes centromeres.bioRxiv : the preprint server for biology · 2025Article
- The dynamic centromere.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Review
- Preserving centromere identity: right amounts of CENP-A at the right place and time.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Review
- Cohesin-mediated stabilization of the CCAN complex at kinetochores in mitosis.Current biology : CB · 2025Article
- High-resolution analysis of human centromeric chromatin.Life science alliance · 2025Article
- Determinants of minor satellite RNA function in chromosome segregation in mouse embryonic stem cells.The Journal of cell biology · 2024Article
- Functional plasticity in chromosome-microtubule coupling on the evolutionary time scale.Life science alliance · 2023Article
- Repression of CENP-A assembly in metaphase requires HJURP phosphorylation and inhibition by M18BP1.The Journal of cell biology · 2023Article
- Nucleosomes in mammalian sperm: conveying paternal epigenetic inheritance or subject to reprogramming between generations?Current opinion in genetics & development · 2023Review
- The Four Causes: The Functional Architecture of Centromeres and Kinetochores.Annual review of genetics · 2022Review
- Molecular conflicts disrupting centromere maintenance contribute to Xenopus hybrid inviability.Current biology : CB · 2022Article
- Composition and organization of kinetochores show plasticity in apicomplexan chromosome segregation.The Journal of cell biology · 2022Article
- The DNA-to-cytoplasm ratio broadly activates zygotic gene expression in Xenopus.Current biology : CB · 2021Article
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- Transgenerational inheritance of centromere identity requires the CENP-A N-terminal tail in the C. elegans maternal germ line.PLoS biology · 2021Article
34 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Eukaryotic cells ensure accurate chromosome segregation in mitosis by assembling a microtubule-binding site on each chromosome called the kinetochore that attaches to the mitotic spindle. The kinetochore is assembled specifically during mitosis on a specialized region of each chromosome called the centromere, which is constitutively bound by >15 centromere-specific proteins. These proteins, including centromere proteins A and C (CENP-A and -C), are essential for kinetochore assembly and proper chromosome segregation. How the centromere is assembled and how the centromere promotes mitotic kinetochore formation are poorly understood. We have used Xenopus egg extracts as an in vitro system to study the role of CENP-C in centromere and kinetochore assembly. We show that, unlike the histone variant CENP-A, CENP-C is not maintained at centromeres through spermatogenesis but is assembled at the sperm centromere from the egg cytoplasm. Immunodepletion of CENP-C from metaphase egg extract prevents kinetochore formation on sperm chromatin, and depleted extracts can be complemented with in vitro-translated CENP-C. Using this complementation assay, we have identified CENP-C mutants that localized to centromeres but failed to support kinetochore assembly. We find that the amino terminus of CENP-C promotes kinetochore assembly by ensuring proper targeting of the Mis12/MIND complex and CENP-K.
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