ArticleEuropean biophysics journal : EBJ2010
Solution NMR mapping of water-accessible residues in the transmembrane beta-barrel of OmpX.
Article in European biophysics journal : EBJ, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Efficient detergent removal using a polydivinylbenzene adsorbent in membrane protein solubilization.Bioprocess and biosystems engineering · 2025Article
- Determining the amphipol distribution within membrane-protein fibre samples using small-angle neutron scattering.Acta crystallographica. Section D, Structural biology · 2018Article
- Perturbations of Native Membrane Protein Structure in Alkyl Phosphocholine Detergents: A Critical Assessment of NMR and Biophysical Studies.Chemical reviews · 2018Review
- FPOP-LC-MS/MS Suggests Differences in Interaction Sites of Amphipols and Detergents with Outer Membrane Proteins.Journal of the American Society for Mass Spectrometry · 2017Article
- The use of amphipols for solution NMR studies of membrane proteins: advantages and constraints as compared to other solubilizing media.The Journal of membrane biology · 2014Article
- Labeling and functionalizing amphipols for biological applications.The Journal of membrane biology · 2014Review
- Application of amphipols for structure-functional analysis of TRP channels.The Journal of membrane biology · 2014Review
- Outer membrane protein F stabilised with minimal amphipol forms linear arrays and LPS-dependent 2D crystals.The Journal of membrane biology · 2014Article
- Synthesis, characterization and applications of a perdeuterated amphipol.The Journal of membrane biology · 2014Article
- How amphipols embed membrane proteins: global solvent accessibility and interaction with a flexible protein terminus.The Journal of membrane biology · 2014Article
- Molecular dynamics simulations of a membrane protein/amphipol complex.The Journal of membrane biology · 2014Article
- Solution behavior and crystallization of cytochrome bc₁ in the presence of amphipols.The Journal of membrane biology · 2014Article
- Long-term stability of a vaccine formulated with the amphipol-trapped major outer membrane protein from Chlamydia trachomatis.The Journal of membrane biology · 2014Article
- Amphipols for each season.The Journal of membrane biology · 2014Review
- Amphipol-mediated screening of molecular orthoses specific for membrane protein targets.The Journal of membrane biology · 2014Article
- Increased immunoaccessibility of MOMP epitopes in a vaccine formulated with amphipols may account for the very robust protection elicited against a vaginal challenge with Chlamydia muridarum.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- NMR-based detection of hydrogen/deuterium exchange in liposome-embedded membrane proteins.PloS one · 2014Article
- Amphipol-assisted folding of bacteriorhodopsin in the presence or absence of lipids: functional consequences.European biophysics journal : EBJ · 2013Article
- Folding and stability of outer membrane protein A (OmpA) from Escherichia coli in an amphipathic polymer, amphipol A8-35.European biophysics journal : EBJ · 2013Article
- Molecular architecture and subunit organization of TRPA1 ion channel revealed by electron microscopy.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The atomic structure of OmpX, the smallest member of the bacterial outer membrane protein family, has been previously established by X-ray crystallography and NMR spectroscopy. In apparent conflict with electrophysiological studies, the lumen of its transmembrane beta-barrel appears too tightly packed with amino acid side chains to let any solute flow through. In the present study, high-resolution solution NMR spectra were obtained of OmpX kept water-soluble by either amphipol A8-35 or the detergent dihexanoylphosphatidylcholine. Hydrogen/deuterium exchange measurements performed after prolonged equilibration show that, whatever the surfactant used, some of the amide protons of the membrane-spanning region exchange much more readily than others, which likely reflects the dynamics of the barrel.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.