ArticlePhysical chemistry chemical physics : PCCP2009
Site-specific dynamic nuclear polarization of hydration water as a generally applicable approach to monitor protein aggregation.
Article in Physical chemistry chemical physics : PCCP, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 46 citations in OpenAlex.
- Micropolarity governs the structural organization of biomolecular condensates.Nature chemical biology · 2024Article
- Toward protein NMR at physiological concentrations by hyperpolarized water-Finding and mapping uncharted conformational spaces.Science advances · 2022Article
- Hyperpolarized water as universal sensitivity booster in biomolecular NMR.Nature protocols · 2022Review
- Deciphering the Structure and Formation of Amyloids in Neurodegenerative Diseases With Chemical Biology Tools.Frontiers in chemistry · 2022Review
- Liquid-Liquid Phase Separation of Tau Driven by Hydrophobic Interaction Facilitates Fibrillization of Tau.Journal of molecular biology · 2021Article
- Narrow equilibrium window for complex coacervation of tau and RNA under cellular conditions.eLife · 2019Article
- Efficient assignment and NMR analysis of an intact virus using sequential side-chain correlations and DNP sensitization.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Surface water retardation around single-chain polymeric nanoparticles: critical for catalytic function?Chemical science · 2016Article
- Protein structural and surface water rearrangement constitute major events in the earliest aggregation stages of tau.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Anomalously Rapid Hydration Water Diffusion Dynamics Near DNA Surfaces.Journal of the American Chemical Society · 2015Article
- Hydration water mobility is enhanced around tau amyloid fibers.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Probing water density and dynamics in the chaperonin GroEL cavity.Journal of the American Chemical Society · 2014Article
- Quantitative cw Overhauser effect dynamic nuclear polarization for the analysis of local water dynamics.Progress in nuclear magnetic resonance spectroscopy · 2013Review
- Overhauser Dynamic Nuclear Polarization-Enhanced NMR Relaxometry.Microporous and mesoporous materials : the official journal of the International Zeolite Association · 2013Article
- Structural insight into proteorhodopsin oligomers.Biophysical journal · 2013Article
- Polarizing agents and mechanisms for high-field dynamic nuclear polarization of frozen dielectric solids.Solid state nuclear magnetic resonance · 2011Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
We present a generally applicable approach for monitoring protein aggregation by detecting changes in surface hydration water dynamics and the changes in solvent accessibility of specific protein sites, as protein aggregation proceeds in solution state. This is made possible through the Overhauser dynamic nuclear polarization (DNP) of water interacting with stable nitroxide spin labels tethered to specific proteins sites. This effect is highly localized due to the magnetic dipolar nature of the electron-proton spin interaction, with >80% of their interaction occurring within 5 A between the unpaired electron of the spin label and the proton of water. We showcase our tool on the aggregation of tau proteins, whose fibrillization is linked to neurodegenerative disease pathologies known as taupathies. We demonstrate that the DNP approach to monitor local changes in hydration dynamics with residue specificity and local contrast can distinguish specific and neat protein-protein packing leading to fibers from non-specific protein agglomeration or precipitation. The ability to monitor tau assembly with local, residue-specific, resolution, under ambient conditions and in solution state will help unravel the mechanism and structural characteristics of the gradual process of tau aggregation into amyloid fibers, which remains unclear to this day.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.