ArticleThe Journal of biological chemistry2009
Identification and functional characterization of cytoplasmic determinants of plasmid DNA nuclear import.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 41 citations in OpenAlex.
- VRK1 co-delivery mitigates DNA clustering by BAF in TFAMoplex transfection.Materials today. Bio · 2025Article
- Mapping cellular processes that determine delivery of plasmid DNA to the nucleus: application in Chinese hamster ovary and human embryonic kidney cells to enhance protein production.Frontiers in bioengineering and biotechnology · 2025Article
- Nuclear Entry of DNA and Transgene Expression in Dividing and Non-dividing Cells.Cellular and molecular bioengineering · 2023Article
- Ultrasound-targeted cationic microbubbles combined with the NFκB binding motif increase SDF-1α gene transfection: A protective role in hearts after myocardial infarction.The Kaohsiung journal of medical sciences · 2022Article
- Engineering of Chinese Hamster Ovary Cells With NDPK-A to Enhance DNA Nuclear Delivery Combined With EBNA1 Plasmid Maintenance Gives Improved Exogenous Transient Reporter, mAb and SARS-CoV-2 Spike Protein Expression.Frontiers in bioengineering and biotechnology · 2021Article
- Synthetic Approaches for Nucleic Acid Delivery: Choosing the Right Carriers.Life (Basel, Switzerland) · 2019Review
- Cytoplasmic transport and nuclear import of plasmid DNA.Bioscience reports · 2017Review
- Ultrasound‑targeted microbubbles combined with a peptide nucleic acid binding nuclear localization signal mediate transfection of exogenous genes by improving cytoplasmic and nuclear import.Molecular medicine reports · 2017Article
- Characterization of exogenous DNA mobility in live cells through fluctuation correlation spectroscopy.Scientific reports · 2015Article
- Live Cell Characterization of DNA Aggregation Delivered through Lipofection.Scientific reports · 2015Article
- Electroporation-mediated gene delivery.Advances in genetics · 2015Article
- On the possible involvement of bovine serum albumin precursor in lipofection pathway.Journal of biosciences · 2014Article
- Proteomic and functional analyses of protein-DNA complexes during gene transfer.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Article
- Exploring the mechanism of plasmid DNA nuclear internalization with polymer-based vehicles.Molecular pharmaceutics · 2012Article
- Membrane and nuclear permeabilization by polymeric pDNA vehicles: efficient method for gene delivery or mechanism of cytotoxicity?Molecular pharmaceutics · 2012Article
- Transcription factor plasmid binding modulates microtubule interactions and intracellular trafficking during gene transfer.Gene therapy · 2012Article
- Improved antibiotic-free plasmid vector design by incorporation of transient expression enhancers.Gene therapy · 2011Article
- Progress and prospects: nuclear import of nonviral vectors.Gene therapy · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
20 authors at 4 institutions in 3 countries.
Funding
Abstract
Import of exogenous plasmid DNA (pDNA) into mammalian cell nuclei represents a key intracellular obstacle to efficient non-viral gene delivery. This includes access of the pDNA to the nuclei of non-dividing cells where the presence of an intact nuclear membrane is limiting for gene transfer. Here we identify, isolate, and characterize, cytoplasmic determinants of pDNA nuclear import into digitonin-permeabilized HeLa cells. Depletion of putative DNA-binding proteins, on the basis of their ability to bind immobilized pDNA, abolished pDNA nuclear import supporting the critical role of cytoplasmic factors in this process. Elution of pDNA-bound proteins, followed by two-dimensional sodium dodecyl polyacrylamide gel electrophoresis identified several candidate DNA shuttle proteins. We show that two of these, NM23-H2, a ubiquitous c-Myc transcription-activating nucleoside diphosphate kinase, and the core histone H2B can both reconstitute pDNA nuclear import. Further, we demonstrate a significant increase in gene transfer in non-dividing HeLa cells transiently transfected with pDNA containing binding sequences from two of the DNA shuttle proteins, NM23-H2 and the homeobox transcription factor Chx10. These data support the hypothesis that exogenous pDNA binds to cytoplasmic shuttle proteins and is then translocated to the nucleus using the minimal import machinery. Importantly, increasing the binding of pDNA to shuttle proteins by re-engineering reporter plasmids with shuttle binding sequences enhances gene transfer. Increasing the potential for exogenously added pDNA to bind intracellular transport cofactors may enhance the potency of non-viral gene transfer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.