ArticleJournal of virological methods2009
Simultaneous detection of respiratory viruses in children with acute respiratory infection using two different multiplex reverse transcription-PCR assays.
Article in Journal of virological methods, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 44 citations in OpenAlex.
- New methods to detect bacterial or viral infections in patients with chronic obstructive pulmonary disease.Expert review of respiratory medicine · 2024Review
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- Development of a multiplex RT-PCR for simultaneous diagnosis of human metapneumovirus (HMPV) and human respiratory syncytial virus (HRSV) from clinical specimens.Virusdisease · 2016Article
- A Melting Curve-Based Multiplex RT-qPCR Assay for Simultaneous Detection of Four Human Coronaviruses.International journal of molecular sciences · 2016Article
- Pentraxin 3 as a clinical marker in children with lower respiratory tract infection.Pediatric pulmonology · 2016Article
- Evaluation of the AdvanSure™ real-time RT-PCR compared with culture and Seeplex RV15 for simultaneous detection of respiratory viruses.Diagnostic microbiology and infectious disease · 2014Article
- Respiratory virus infections in hospitalized children and adults in Lao PDR.Influenza and other respiratory viruses · 2013Article
- Development of a multiplex one step RT-PCR that detects eighteen respiratory viruses in clinical specimens and comparison with real time RT-PCR.Journal of virological methods · 2013Article
- Comparative evaluation of six commercialized multiplex PCR kits for the diagnosis of respiratory infections.PloS one · 2013Article
- Respiratory viral pathogens associated with lower respiratory tract disease among young children in the highlands of Papua New Guinea.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2012Article
- Lower respiratory infections among hospitalized children in New Caledonia: a pilot study for the Pneumonia Etiology Research for Child Health project.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2012Article
- Differing clinical characteristics between influenza strains among young healthy adults in the tropics.BMC infectious diseases · 2012Article
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- Evaluation of multiple commercial molecular and conventional diagnostic assays for the detection of respiratory viruses in children.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2011Article
- Detection of respiratory viruses on air filters from aircraft.Letters in applied microbiology · 2011Article
- Development and evaluation of a four-tube real time multiplex PCR assay covering fourteen respiratory viruses, and comparison to its corresponding single target counterparts.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2011Article
- Simultaneous detection of influenza A, influenza B, and respiratory syncytial viruses and subtyping of influenza A H3N2 virus and H1N1 (2009) virus by multiplex real-time PCR.Journal of clinical microbiology · 2011Article
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Corrections and comments
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Authors and funding
11 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A 4-tube multiplex RT-PCR (mRT-PCR), which showed higher sensitivity over conventional methods, was previously developed for the diagnosis of 14 viral pathogens of the respiratory tract. Herein the mRT-PCR was compared to the commercial Luminex mPCR-microsphere flow cytometry assay (Resplex II) which allows the detection of 12 different viruses. Eleven different viruses were identified in 91 nasopharyngeal swabs of children with acute respiratory infection, influenza A (IAV) and B, respiratory syncytial virus (RSV), human rhinovirus (hRhV), human echovirus, parainfluenza viruses (PIV) 1, 2, 3 and 4, human metapneumovirus (hMPV), and human coronavirus NL63. The results of the two techniques showed 53 and 40 positive patients by the Resplex II assay and mRT-PCR, respectively, with a concordance in 35 positive and 33 negative patients (74.7%). Individual RT-PCR tests were performed to control viruses not simultaneously detected by the two multiplex assays. The major virus misdiagnosed by mRT-PCR was IAV whereas the major viruses misdiagnosed by Resplex II were PIV1, 3 and 4. The mRT-PCR remains a simple, rapid, and specific assay for the specific detection of respiratory viruses, and can be easily implemented with standards in clinical laboratories at a low cost.
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