ArticleBMC molecular biology2009
Multiple histone modifications in euchromatin promote heterochromatin formation by redundant mechanisms in Saccharomyces cerevisiae.
Article in BMC molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 31 citations in OpenAlex.
- Evolution of a distinct chromatin regulatory landscape in brown algae.Nature ecology & evolution · 2026Article
- BAP1 constrains pervasive H2AK119ub1 to control the transcriptional potential of the genome.Genes & development · 2021Article
- Metabolic regulation of telomere silencing by SESAME complex-catalyzed H3T11 phosphorylation.Nature communications · 2021Article
- Yeast epigenetics: the inheritance of histone modification states.Bioscience reports · 2019Review
- Antisense transcriptional interference mediates condition-specific gene repression in budding yeast.Nucleic acids research · 2018Article
- Ten principles of heterochromatin formation and function.Nature reviews. Molecular cell biology · 2018Review
- Spurious transcription factor binding: non-functional or genetically redundant?BioEssays : news and reviews in molecular, cellular and developmental biology · 2014Article
- Article
- Set5 and Set1 cooperate to repress gene expression at telomeres and retrotransposons.Epigenetics · 2014Article
- Sirt1 suppresses RNA synthesis after UV irradiation in combined xeroderma pigmentosum group D/Cockayne syndrome (XP-D/CS) cells.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Mechanism for epigenetic variegation of gene expression at yeast telomeric heterochromatin.Genes & development · 2012Article
- Deficiency of H3K79 histone methyltransferase Dot1-like protein (DOT1L) inhibits cell proliferation.The Journal of biological chemistry · 2012Article
- A dual role of H4K16 acetylation in the establishment of yeast silent chromatin.The EMBO journal · 2011Article
- Regulation of the DNA damage response and gene expression by the Dot1L histone methyltransferase and the 53Bp1 tumour suppressor.PloS one · 2011Article
- Dot1 binding induces chromatin rearrangements by histone methylation-dependent and -independent mechanisms.Epigenetics & chromatin · 2011Article
- Chromatin structure following UV-induced DNA damage-repair or death?International journal of molecular sciences · 2011Review
- The inheritance of histone modifications depends upon the location in the chromosome in Saccharomyces cerevisiae.PloS one · 2011Article
- The Saccharomyces cerevisiae anaphase-promoting complex interacts with multiple histone-modifying enzymes to regulate cell cycle progression.Eukaryotic cell · 2010Article
- The ubiquitin-conjugating enzyme HR6B is required for maintenance of X chromosome silencing in mouse spermatocytes and spermatids.BMC genomics · 2010Article
- Epigenome manipulation as a pathway to new natural product scaffolds and their congeners.Natural product reports · 2010Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMethylation of lysine 79 on histone H3 by Dot1 is required for maintenance of heterochromatin structure in yeast and humans. However, this histone modification occurs predominantly in euchromatin. Thus, Dot1 affects silencing by indirect mechanisms and does not act by the recruitment model commonly proposed for histone modifications. To better understand the role of H3K79 methylation gene silencing, we investigated the silencing function of Dot1 by genetic suppressor and enhancer analysis and examined the relationship between Dot1 and other global euchromatic histone modifiers.
resultWe determined that loss of H3K79 methylation results in a partial silencing defect that could be bypassed by conditions that promote targeting of Sir proteins to heterochromatin. Furthermore, the silencing defect in strains lacking Dot1 was dependent on methylation of H3K4 by Set1 and histone acetylation by Gcn5, Elp3, and Sas2 in euchromatin. Our study shows that multiple histone modifications associated with euchromatin positively modulate the function of heterochromatin by distinct mechanisms. Genetic interactions between Set1 and Set2 suggested that the H3K36 methyltransferase Set2, unlike most other euchromatic modifiers, negatively affects gene silencing.
conclusionOur genetic dissection of Dot1's role in silencing in budding yeast showed that heterochromatin formation is modulated by multiple euchromatic histone modifiers that act by non-overlapping mechanisms. We discuss how euchromatic histone modifiers can make negative as well as positive contributions to gene silencing by competing with heterochromatin proteins within heterochromatin, within euchromatin, and at the boundary between euchromatin and heterochromatin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.