ArticleJournal of proteome research2009
Analysis of vaccinia virus-host protein-protein interactions: validations of yeast two-hybrid screenings.
Article in Journal of proteome research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed, 62 citations in OpenAlex.
- Pooled PPIseq: Screening the SARS-CoV-2 and human interface with a scalable multiplexed protein-protein interaction assay platform.PloS one · 2025Article
- Host-pathogen protein interaction studies: quality control of cDNA libraries using nanopore sequencing.PloS one · 2025Article
- The nuclear localization signal of monkeypox virus protein P2 orthologue is critical for inhibition of IRF3-mediated innate immunity.Emerging microbes & infections · 2024Article
- Fatal attraction: The roles of ribosomal proteins in the viral life cycle.Wiley interdisciplinary reviews. RNA · 2021Review
- Review
- Protein-protein interactions of human viruses.Seminars in cell & developmental biology · 2020Review
- Intraviral interactome of Chikungunya virus reveals the homo-oligomerization and palmitoylation of structural protein TF.Biochemical and biophysical research communications · 2019Article
- Oncoviruses Can Drive Cancer by Rewiring Signaling Pathways Through Interface Mimicry.Frontiers in oncology · 2019Article
- Common Nodes of Virus-Host Interaction Revealed Through an Integrated Network Analysis.Frontiers in immunology · 2019Article
- Interface-Based Structural Prediction of Novel Host-Pathogen Interactions.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Cellular interactome analysis of vaccinia virus K7 protein identifies three transport machineries as binding partners for K7.Virus genes · 2017Article
- Structural host-microbiota interaction networks.PLoS computational biology · 2017Review
- Network analysis reveals common host protein/s modulating pathogenesis of neurotropic viruses.Scientific reports · 2016Article
- Stress Beyond Translation: Poxviruses and More.Viruses · 2016Review
- The Vpu-interacting Protein SGTA Regulates Expression of a Non-glycosylated Tetherin Species.Scientific reports · 2016Article
- Integrative proteomics to understand the transmission mechanism of Barley yellow dwarf virus-GPV by its insect vector Rhopalosiphum padi.Scientific reports · 2015Article
- Simultaneous Quantification of Viral Antigen Expression Kinetics Using Data-Independent (DIA) Mass Spectrometry.Molecular & cellular proteomics : MCP · 2015Article
- Deletion of the vaccinia virus N2L gene encoding an inhibitor of IRF3 improves the immunogenicity of modified vaccinia virus Ankara expressing HIV-1 antigens.Journal of virology · 2014Article
- Exploration of panviral proteome: high-throughput cloning and functional implications in virus-host interactions.Theranostics · 2014Article
- Vaccinia virus protein N2 is a nuclear IRF3 inhibitor that promotes virulence.The Journal of general virology · 2013Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
Vaccinia virus, a large double-stranded DNA virus, is the prototype of the Orthopoxvirus genus, which includes several pathogenic poxviruses of humans, such as monkeypox virus and variola virus. Here, we report a comprehensive yeast two-hybrid (Y2H) screening for the protein-protein interactions between vaccinia and human proteins. A total of 109 novel vaccinia-human protein interactions were detected among 33 viral proteins. To validate subsets of those interactions, we constructed an ORFeome library of vaccinia virus strain WR using the Gateway plasmid cloning system. By co-expressing selected vaccinia and host proteins in a variety of expression systems, we found that at least 17 of the Y2H hits identified between vaccinia and human proteins can be verified by independent methods using GST pull-down assays, representing a 63% validation rate for the Y2H hits examined (17/27). Because the cloned ORFs are conveniently transferable from the entry vectors to various destination expression vectors, the vaccinia ORFeome library will be a useful resource for future high-throughput functional proteomic experiments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.