ArticleInflammatory bowel diseases2009
Defect in CEACAM family member expression in Crohn's disease IECs is regulated by the transcription factor SOX9.
Article in Inflammatory bowel diseases, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 48 citations in OpenAlex.
- Transcriptomic analysis identifies diagnostic genes in polycystic ovary syndrome and periodontitis.European journal of medical research · 2024Article
- Reactive oxygen species-responsive nanocarrier ameliorates murine colitis by intervening colonic innate and adaptive immune responses.Molecular therapy : the journal of the American Society of Gene Therapy · 2023Article
- Patient-derived Colonoids From Disease-spared Tissue Retain Inflammatory Bowel Disease-specific Transcriptomic Signatures.Gastro hep advances · 2023Article
- CEACAMS 1, 5, and 6 in disease and cancer: interactions with pathogens.Genes & cancer · 2023Review
- Modulation of macrophage inflammatory function through selective inhibition of the epigenetic reader protein SP140.BMC biology · 2022Article
- Fecal multi-omics analysis reveals diverse molecular alterations of gut ecosystem in COVID-19 patients.Analytica chimica acta · 2021Article
- Regulation of CEACAM Family Members by IBD-Associated Triggers in Intestinal Epithelial Cells, Their Correlation to Inflammation and Relevance to IBD Pathogenesis.Frontiers in immunology · 2021Article
- CEACAM1 regulates CD8Gut microbes · 2020Article
- Site-specific gene expression profiling as a novel strategy for unravelling keloid disease pathobiology.PloS one · 2017Article
- SOX9 indirectly regulates CEACAM1 expression and immune resistance in melanoma cells.Oncotarget · 2016Article
- Interleukin-6 trans-signaling increases the expression of carcinoembryonic antigen-related cell adhesion molecules 5 and 6 in colorectal cancer cells.BMC cancer · 2015Article
- MicroRNA-146a constrains multiple parameters of intestinal immunity and increases susceptibility to DSS colitis.Oncotarget · 2015Article
- Characterizing CEACAM5 interaction with CD8α and CD1d in intestinal homeostasis.Mucosal immunology · 2014Article
- Article
- Balance of CD8+ CD28+ / CD8+ CD28- T lymphocytes is vital for patients with ulcerative colitis.Digestive diseases and sciences · 2013Article
- The possible roles of OPN-regulated CEACAM1 expression in promoting the survival of activated T cells and the apoptosis of oral keratinocytes in oral lichen planus patients.Journal of clinical immunology · 2011Article
- CEACAM6 gene variants in inflammatory bowel disease.PloS one · 2011Article
- Notch-1 signaling regulates intestinal epithelial barrier function, through interaction with CD4+ T cells, in mice and humans.Gastroenterology · 2011Article
- Intestinal epithelial cells in inflammatory bowel diseases.World journal of gastroenterology · 2010Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
Abstract
backgroundCEACAM1, CEACAM5, and CEACAM6 represent 3 of the CEACAM (carcinoembryonic antigen-related cell adhesion molecule) subfamily members expressed on intestinal epithelial cells (IECs). Deficiency in their expression, as seen in inflammatory bowel disease (IBD), results in the lack of activation of CD8+ regulatory T cells in the mucosa. Since CEACAM expression was shown to be regulated by the transcription factor SOX9, we sought to determine whether the defect in CEACAM expression in IBD was related to aberrant SOX9 expression.
methodsIECs and lamina propria lymphocytes (LPLs) were freshly isolated from colonic tissues. T84 and HT29 16E cells were cocultured with LPLs. SOX9 and CEACAM subfamily member expression was assessed by real-time polymerase chain reaction (PCR), Western blot, immunohistochemistry, and immunofluorescence.
resultsIn Crohn's disease (CD) but not in ulcerative colitis (UC), a significant reduction in mRNA and protein expression for CEACAM1 and 5 was noted; in contrast, no difference in SOX9 mRNA expression was seen. However, nuclear SOX9 immunostaining was increased in CD IECs. Furthermore, SOX9 protein was reduced in the cytoplasm of LPL-stimulated T84 and HT29 16E cells, while CEACAM5 expression was increased.
conclusionsThe defect in CEACAM family members in CD IECs appears to be related to the aberrant nuclear localization of SOX9. Changes in SOX9 expression in the CD mucosa relate to the local microenvironment and altered IEC:LPL crosstalk.
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