ArticlePlant molecular biology2009
AtObgC, a plant ortholog of bacterial Obg, is a chloroplast-targeting GTPase essential for early embryogenesis.
Article in Plant molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 42 citations in OpenAlex.
- The structure-function analysis of Obg-like GTPase proteins along the evolutionary tree from bacteria to humans.Genes to cells : devoted to molecular & cellular mechanisms · 2022Review
- Oryza sativa ObgC1 Acts as a Key Regulator of DNA Replication and Ribosome Biogenesis in Chloroplast Nucleoids.Rice (New York, N.Y.) · 2021Article
- Identification of Chloroplast Envelope Proteins with Critical Importance for Cold Acclimation.Plant physiology · 2020Article
- Chloroplast vesicle transport.Photosynthesis research · 2018Review
- RiceG3 (Bethesda, Md.) · 2018Article
- Structural and biochemical analysis ofThe Journal of biological chemistry · 2017Article
- BcMtg2 is required for multiple stress tolerance, vegetative development and virulence in Botrytis cinerea.Scientific reports · 2016Article
- Leaf patterning of Clivia miniata var. variegata is associated with differential DNA methylation.Plant cell reports · 2016Article
- Evidence for lateral gene transfer (LGT) in the evolution of eubacteria-derived small GTPases in plant organelles.Frontiers in plant science · 2014Article
- The puzzle of chloroplast vesicle transport - involvement of GTPases.Frontiers in plant science · 2014Review
- Article
- The universally conserved prokaryotic GTPases.Microbiology and molecular biology reviews : MMBR · 2011Review
- Biogenesis of thylakoid networks in angiosperms: knowns and unknowns.Plant molecular biology · 2011Review
- Identification of nuclear genes encoding chloroplast-localized proteins required for embryo development in Arabidopsis.Plant physiology · 2011Article
- Genes co-expressed with CPSAR1 identified using ATTED-II.Plant signaling & behavior · 2010Article
- Indispensable Roles of Plastids in Arabidopsis thaliana Embryogenesis.Current genomics · 2010Article
- Megadalton complexes in the chloroplast stroma of Arabidopsis thaliana characterized by size exclusion chromatography, mass spectrometry, and hierarchical clustering.Molecular & cellular proteomics : MCP · 2010Article
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Authors and funding
15 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Obg is a ribosome-associated GTPase essential for bacterial viability and is conserved in most organisms, from bacteria to eukaryotes. Obg is also expressed in plants, which predicts an important role for this molecule in plant viability; however, the functions of the plant Obg homologs have not been reported. Here, we first identified Arabidopsis AtObgC as a plant chloroplast-targeting Obg and elucidated its molecular biological and physiological properties. AtObgC encodes a plant-specific Obg GTPase that contains an N-terminal region for chloroplast targeting and has intrinsic GTP hydrolysis activity. A targeting assay using a few AtObgC N-terminal truncation mutants revealed that AtObgC localizes to chloroplasts and its transit peptide consists of more than 50 amino acid residues. Interestingly, GFP-fused full-length AtObgC exhibited a punctate staining pattern in chloroplasts of Arabidopsis protoplasts, which suggests a dimerization or multimerization of AtObgC. Moreover, its Obg fold was indispensable for the generation of the punctate staining pattern, and thus, was supposed to be important for such oligomerization of AtObgC by mediating the protein-protein interaction. In addition, the T-DNA insertion AtObgC null mutant exhibited an embryonic lethal phenotype that disturbed the early stage of embryogenesis. Altogether, our results provide a significant implication that AtObgC as a chloroplast targeting GTPase plays an important role at the early embryogenesis by exerting its function in chloroplast protein synthesis.
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